vostok
Well-Known Member
In a survey conducted in Cannabis sativa L. (cannabis) authorized farms in Israel,
plants showed disease symptoms characteristic of nutrition deprivation.
Interveinal chlorosis, brittleness, and occasional necrosis were observed in older leaves.
Next generation sequencing analysis of RNA extracted from symptomatic
leaves revealed the presence of lettuce chlorosis virus (LCV), a crinivirus
that belongs to the Closteroviridae family.
The complete viral genome sequence was obtained using RT-PCR and
Rapid Amplification of cDNA Ends (RACE) PCR followed by Sanger sequencing.
The two LCV RNA genome segments shared 85–99% nucleotide sequence identity
with LCV isolates from GenBank database.
The whitefly Bemisia tabaci Middle Eastern Asia Minor1 (MEAM1) biotype transmitted
the disease from symptomatic cannabis plants to un-infected ‘healthy’
Cannabis, Lettuce, and Periwinkles.
Shoots from symptomatic cannabis plants, used for plant propagation, constituted
a primary inoculum of the disease. To the best of our knowledge, this is the first
report of cannabis plant disease caused by LCV
https://www.mdpi.com/1999-4915/11/9/802/htm
Full pfd in support: https://www.mdpi.com/1999-4915/11/9/802/pdf
Shits happening Now: Stay Sharp
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