Mycology86
Member
Can anybody offer me some better expertise? I'm into much more "technical," (i think it the more "proper,") forms of propagating different species of mushroom cultivars now, I'm working on my final leg of my research and experimentation. I have battled low humidities, low temperatures, and I've tried so far to maximize flush yields, maximize actual occurrences of multiple flushes, and have been trying to establish the possibility of "Perpetual Flushings," as i termed it, 
while simultaneously shortening the time between flushes. Meanwhile, i always try to minimize wasted space as best I can. I purposely put myself directly in over my head with my research because it causes me to focus harder in between growth phases, theoretical contaminations, or power outages. When I force myself beyond.my mental threshold of anxiety and insomnia, its only then will I persevere and actually thrive, until mental breakthrough or meltdown. Anyways... I started out using wild fall oysters I found actually in January. I printed them, syringed the spores, inoculated some corrugated cardboard and seperated rhizomorphs into long containers, short boxy containers, and a few hanging, clear sterile meat bags you put your packages of raw meat into at the grocery store. I used a mixture of pasteurized straw, coffee grounds, and ground up aspen logs. Both the logs and straw were harvested naturally and therefore were already in the process of degredation (decomposition). It seems the mycelium took over the substrate more easily than if I used fresh, not rotting, ingredients... I haven't had trouble with contamination at all yet, a flake or two of trachederma both very mild. But the mycelium blasted right thru in the flatter containers and smaller containers, but not the bags. My hypothesis is that the decomposition of the straw and aspen sucked precious nitrogen and carbohydrates from my substrate, causing the mycelium to reach their filamentous fibers out of the substrate. I'm working on degrading nutrients out of common pet supplies (reptile nutrient dust and cricket food. Both with a plethora of essential nutrients and amino acids and proteins. Should I case them after I breakdown the nutrient boost into water soluble form???? And I also innoculated. Some bfr/verm cakes with pink buffalo. My planexperiment was to use a combo of rotting straw, chicken manure(decomposed ) and a secret mixture of slightly nutrient-deprived materials along with some hydrated lime, but the chicken manure is already slightly buffered to be on the basic side of the Ph scale. Will this kill/damage my mycelium? I am against using just the cake tell because it is a waste of mycelium. I will gently break them up and lay them out on my blend of materials, which will itself be on a layer of Verm. Then I will cover myc with blended substrate and put bak in the dark without fresh air. How thick of layers of each should I possibly try?? And with this mush mash of nutrients and raw materials, can you predict an approximate timeframe before I start their fruiting stage?? Thank you if you choose to advise me, it is much appreciated, and i apologize for the lenghty procedural ramblings.I am a scientist, and I am just trying to be thorough. Enough so that someone may be able to incision my dream the way I see it??? 
while simultaneously shortening the time between flushes. Meanwhile, i always try to minimize wasted space as best I can. I purposely put myself directly in over my head with my research because it causes me to focus harder in between growth phases, theoretical contaminations, or power outages. When I force myself beyond.my mental threshold of anxiety and insomnia, its only then will I persevere and actually thrive, until mental breakthrough or meltdown. Anyways... I started out using wild fall oysters I found actually in January. I printed them, syringed the spores, inoculated some corrugated cardboard and seperated rhizomorphs into long containers, short boxy containers, and a few hanging, clear sterile meat bags you put your packages of raw meat into at the grocery store. I used a mixture of pasteurized straw, coffee grounds, and ground up aspen logs. Both the logs and straw were harvested naturally and therefore were already in the process of degredation (decomposition). It seems the mycelium took over the substrate more easily than if I used fresh, not rotting, ingredients... I haven't had trouble with contamination at all yet, a flake or two of trachederma both very mild. But the mycelium blasted right thru in the flatter containers and smaller containers, but not the bags. My hypothesis is that the decomposition of the straw and aspen sucked precious nitrogen and carbohydrates from my substrate, causing the mycelium to reach their filamentous fibers out of the substrate. I'm working on degrading nutrients out of common pet supplies (reptile nutrient dust and cricket food. Both with a plethora of essential nutrients and amino acids and proteins. Should I case them after I breakdown the nutrient boost into water soluble form???? And I also innoculated. Some bfr/verm cakes with pink buffalo. My planexperiment was to use a combo of rotting straw, chicken manure(decomposed ) and a secret mixture of slightly nutrient-deprived materials along with some hydrated lime, but the chicken manure is already slightly buffered to be on the basic side of the Ph scale. Will this kill/damage my mycelium? I am against using just the cake tell because it is a waste of mycelium. I will gently break them up and lay them out on my blend of materials, which will itself be on a layer of Verm. Then I will cover myc with blended substrate and put bak in the dark without fresh air. How thick of layers of each should I possibly try?? And with this mush mash of nutrients and raw materials, can you predict an approximate timeframe before I start their fruiting stage?? Thank you if you choose to advise me, it is much appreciated, and i apologize for the lenghty procedural ramblings.I am a scientist, and I am just trying to be thorough. Enough so that someone may be able to incision my dream the way I see it??? 
