Highest THC Content?

stonedmetalhead1

Well-Known Member
I understand all of that so on average how many tests are you running on each submitted sample of bud when you give someone results?
 

Matt Rize

Hashmaster
ALSO A QUESTION TO THE SCIENTIST (if any are out there) = "WHICH STRAIN HAS THE HIGHEST THCV%?!?!?"


I probably should have started another thread with these questions, but this one seems to still be pretty active. Also, most people would word the question for the answer I'm looking for like hazey grapes did.

Hopefully this can stay an spirited/intelligent/positive discussion amongst a people of a united cause, and we leave the spiteful/ignorant/negative debating to the politicians impossible to unite egoistic agendas ;)
Thanks for coming in and clearing these things up.
The only high THCV "strain" that I know of is a Jack the Ripper pheno. google will bring you to info on that pheno. And the breeder SUBCOOL is a moderator here with his own subforum in the organic forum.
 

Matt Rize

Hashmaster
From the chromatogram of the sample extract alone, you're right, it is not possible to calculate the percentage by weight. However when combining this information with the chromatograms of the standards and the weight of the sample from which the extract is made, these calculations can be made according to the formula I previously posted.
Thank you again. rize up!
 

Matt Rize

Hashmaster
I'm familiar with the SC Labs series of videos. They've partnered up with WeedMaps so they've got access to more resources than we do but we plan on producing similar ones targeted towards patients in Michigan.

Unfortunately, there are no commerical standards available for THCV and many of the other minor cannabinoids, however we have made requests with our suppliers to provide these compounds for us. The only other option is to custom purify these compounds in house however this would require the use of a mass spectrometer, LC, and fraction collector. We plan on doing this in the future but lack the mass spec at the moment.
Can you make your own THCV standard using preparative HPLC?
You using Cerilliant standards?


VVVVV Don't assume. What he is doing is called trolling. He is only looking to prove his preconceived ideas about cannabinoid analysis by peppering you with repetitive/short/leading questions. Notice, you clearly answered his question already, and he asked it again slightly rephrased, classic troll move. He is trying to wear you out, he is a long distance poster, not much substance, but you can't get the last word. Don't let him get to you. Thanks again for dropping the much needed knowledge, especially that simple formula for calculating %. I always have my best buds tested and my #s come up on the high end.
Getting back to your original question, I assume that you're interested in the variability of biological replicates. ...
 
Can you make your own THCV standard using preparative HPLC?
You using Cerilliant standards?


VVVVV Don't assume. What he is doing is called trolling. He is only looking to prove his preconceived ideas about cannabinoid analysis by peppering you with repetitive/short/leading questions. Notice, you clearly answered his question already, and he asked it again slightly rephrased, classic troll move. He is trying to wear you out, he is a long distance poster, not much substance, but you can't get the last word. Don't let him get to you. Thanks again for dropping the much needed knowledge, especially that simple formula for calculating %. I always have my best buds tested and my #s come up on the high end.


We've used standards from both Lipomed & Restek. Restek seems to be the way to go for now. Yes, it is possible to make your own THCV standard using preparative chromatography, but first you've got to find plant material rich in this compound. Also, you'd want a mass spec to be able to confirm the compound you have isolated is the one you intended and to rule out the presence of any background contaminants.
 

RawBudzski

Well-Known Member
20-25% thc strain grown correctly will make you one happy camper. Remember only in perfect conditions will it be that high.
 

Matt Rize

Hashmaster
We've used standards from both Lipomed & Restek. Restek seems to be the way to go for now. Yes, it is possible to make your own THCV standard using preparative chromatography, but first you've got to find plant material rich in this compound. Also, you'd want a mass spec to be able to confirm the compound you have isolated is the one you intended and to rule out the presence of any background contaminants.
For sure. Steep Hill Lab, Im fairly certain, has done this already using the high THCV Jack the Ripper phenotype.
THCV is supposedly the next miracle diet "drug" because it decreases appetite while increasing a sense of well being. Have you heard of this?
 

Matt Rize

Hashmaster
20-25% thc strain grown correctly will make you one happy camper. Remember only in perfect conditions will it be that high.
Its all about the terps. The more I know about cannabinoids and cannabis, the more I know that its not the cannabinoids at all that we care about. Its the terpenoids, flavonoids, esters ect that make up the actual affects. The favorite OGs test way low, Larry OG typically around 12%. Its all about the alpha-pinene in lemony strains, which acts to increase the affects of THC. The difference between agonist terpenoids, and antagonist terpenoids, are what we really care about. But cannabis science is not advanced enough at this stage to be able to look at cannabis from this perspective. So we focus on the big three cannabinoids because they are more straight forward.

At this point in cannabis science, testing a bud by smoking it tells us way more about the affects than testing for cannabinoids. Unless we are specifically looking for molecules other than THC, which is less than 2% of tests.
 

RawBudzski

Well-Known Member
My LA OG Kush is a 25% thc strain, though I am not sure if I am hitting that mark.
My tahoe OG kush is also a 22%+ thc strain.

mothers of my clones are the legit lab tested clones here in cali. drove 80 miles north to pick these babies up from the clone zone.
 

Jar Man

Active Member
Reading this thread shows me how inconsistently screwed up the whole THC % issue is. Because there's no real standard of measure and other reports by the experts claiming how easy it is get differing results confirms how meaningless the figure is. The problem is only exasperated by seedbanks and growers who want to flaunt their superior self image or boost profits. The only accurate method is personal side-by-side smoking comparisons. To hold your own THC Cup showdown of strains.
 

Dan Kone

Well-Known Member
Reading this thread shows me how inconsistently screwed up the whole THC % issue is. Because there's no real standard of measure and other reports by the experts claiming how easy it is get differing results confirms how meaningless the figure is. The problem is only exasperated by seedbanks and growers who want to flaunt their superior self image or boost profits. The only accurate method is personal side-by-side smoking comparisons. To hold your own THC Cup showdown of strains.
Any results posted by someone who is selling seeds can not be trusted.
 

GreenChile

Active Member
I think we all need to grow out our own personal "super dank" strains and have a good ol fashion smoke out :)
We should all meet in Vegas and see who's is bigger :)
I say Halloween in Vegas, everyone brings what they got and it has to be certified to atleast 25%
Winner get my girlfriend (shes hot and she smells like coconut) and her dog! Haha
 
From a chemistry stand-point it makes sense to probably calculate total THC as a function of resin. So if a thousand glands say masses to a total of 1g of resin and the GC-MS says that THC represents 25% of the resin, this is the total THC % content. The misleading part of the question is that it negates the fact that it may take 2 grams of dry bud to make 1 g of resin for one strain versus another that may only take 1.5 grams of dry bud for the same mass of finished resin. Therefore, if one considers that the more efficient plant (plant 25% resin, 1.0 gResin/1.5 gBud versus 25% resin, 1.0gResin/2.0gBud), assuming they have the same THC % content in this model, one can see how the original question of what has the highest THC content really is only arbitrary.
 
If the industry was smart what we would do is actually choose a very general strain that is readily available and use it as a secondary standard for THC content in the resin and the total amount of resin produced as a function of mass of the plant versus age from say the top 10% of the mass range of the plant at harvest at say something like 60 days. This could be used to compare the THC peak, or others for that matter, and a standard could be set. Say we used AK-47, from clone topped three times veg'd for 30 days under a 150 W bulb. Any other plants that would be grown would be reported against this strain. So AK-47 THC content would be equal to 1...something weaker might be .9....AK-47 resin to mass ratio could also be reporter where it would also be equal to one and a plant that produced more could be valued at say, 1.1. SO finding plants that have the ratios greater than 1 to some good standard would significantly help add or label a relative score to the plants we label and spread into the market as superior when they actually may not be. It is unfortunate that many strains entering the market really are not pegged to anything and even worse this is not real general catalogue that is accessible to serious growers and individuals who desire to take the strains to the next level. This and significantly other factors need to be considered when we begin to manufacture so many different strains with not history of their lineage.
 

sgt john

Well-Known Member
I thought we were doing a strain review? Check it out y'all, I don't know about the rest of you but I come to this site to research marijuana. Why, because I believe that many people on this site have first hand knowledge of growing marijuana and have EXPERIENCE that I can learn from.

What I don;t do is come to this site to trade political insight, or to discuss military affairs with those people who do not have first hand knowledge of the subject. Why? Because most people are talking out their ass and have no clue wtf they're speaking on.

I think we need to find some weed that will help a mutherFuker remember wtf they're talking about.


I couldn't put it better words, and Jack Diesel and Kushberry has the high, and great flavor.
 
I'm starting a grow here with some different strains (I hate that I do multiple strains, but can't stop myself;) I have some old fav's, but try to add some new rare strains too and may mother a few out.I was going to grow Girl Scout Cookie's since a clone friend of mine just got some in, but decided to wait and go with an experimental round. Animal Cookies is out now too. Tried looking for Blood Diamond, was interested in Purple Mango, but settled on:

I've got some new crosses - Lucid Dream (Amnesia x Blue Dream), Ken's Dream ( Ken's cut Grand Daddy Purples x Blue Dream), Black Diamond (Blackberry Kush x Diamond OG), and some cannasseur strains - GOO* (not afgoo! this is the rare hard to find sativa GOO which is a top contestant in some local cups... cannasseur strain for sure!!!), JV86 (won 2 local contests in a row now votes by both the judges and attendees choice!!) and a few classic favorites - Kryptonite, Mazar, & C99.

I use 1k watts, soil, a big nUt & tea blend, sealed room with CO2 & AC/Dehumid.....blah blah nerd grower right... ask if you want to know more.

I will probably get into mothering and cloning on this grow too.... wonder which ones would be the best keepers....?

This will def be a tough grow! I always say just 1 strain at a time for ease, and no more than 1 strain per light!!! I'm really gonna test my skillz on this one. I've never kept a journal, but was considering it on this one.... Should be chalked full of some good, bad, funny, (hope not sad), but over all very educational moments.

May even need some help if there are some other experienced growers out there who want to follow....
 

trophy1

Well-Known Member
The strongest strain I have is Anesthesia by Pyramid. The breeder says its 17 %. It is stronger than Tangerine Dream which is suppose to be 25% and the TD is pretty Kick ass but Anesthesia is still the potency queen. I also have White Rhino, Strawberry Cough, Space and Black Jack, these are all good but not in the same league as The Anesthesia and TD.
 
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