Strain Isolation/Master culture: Can this be done at home?

I have been considering doing strain isolation but I wonder if anyone has attempted this at home or does it have to be in a lab? I watched a video "Let's Grow Mushrooms" and this guy went all out and made his closet his working lab.
Is it possible to convert a 4x4 indoor greenhouse into one of these labs? I guess that I just do not know the process very well. Laminar flow hood, are they important? I have taken college chemistry but that is not really helpful here lol. I am looking to get past multi-spore inoculation.

check out paul stamets books...

Easily done at home. Where the fuck is canndo

rory420420 said:

check out paul stamets books...

Click to expand...

I like Paul Stamet. I think he lives jn the Pacific NW. Good climate for mycellium! I watched a couple of his seminars.

Canndo is flat on his back looking up at the ceiling.

You do not need a lab, glove box works fine. Split a fresh mushroom in half, use sterile instruments. Try to get a filiment from th point where the cap and stem meet, pick your biggest fruit and spray it with lysol befire you split it.
Dont use heat. Just have plenty of replacement scalples. One per dish. Mea works best. Dont worry if your filament is blue, it will recover. Make your agar about 10 percent more water than instructed so the finished agar is a tad more moist.

Incubate as you would with spores, in the hugh seventies.

Good luck.