Apical Bud
Well-Known Member
Hi there. I recently got some flower tested at a local lab (I live in Washington so we have cannabis testing labs around) and it came back with a CBD to THC ratio of 9:5. I was under the impression that the ratios should be 1:~0 or 1:1 or ~0:1.
I started this two years ago with an ACDC and a pre98 bubba. I bred the offspring with each other, assuming each would have a 1:1 ratio (one CBD gene from mom, one THC from dad). Then I bred a male and a female from that first generation and applied the alkaline alcohol trick to determine which had CBD. At that point I believed that if I bred a CBD+ male with a CBD+ female I had a good chance of getting some CBD-only seeds.
So I planted 4 of these seeds and all of them happened to be female*. Of these, three tested positive for CBD.
So far, so good! I could assume that half of these three would be 1:1 and half would be all CBD. Well, I "tested" my favorite one, which seemed to get me the least high. The lab came back with 7.7 CBD and 4.3 THC (I cut it off 3-4 weeks into flower). That's about 9/5. This doesn't follow with the neat ratio framework that I imagined.
Does anyone know how to explain this?
I am wondering if I have a tri or tetraploid plant?
*The fact that I got 4/4 females makes me wonder if maybe the mother hermmed and pollinated herself.
Thanks for reading, and I look forward to nerding out.
https://www.genetics.org/content/163/1/335
2) https://www.researchgate.net/figure/Chemotypes-and-CBD-THC-ratios-found-within-each-of-the-accessions-tested_tbl1_225148108 It looks like the ratios aren't as cut and dry as I thought. I don't know how the first link would explain this second one, but here is a list of CBD:THC ratios that have been tested.
I started this two years ago with an ACDC and a pre98 bubba. I bred the offspring with each other, assuming each would have a 1:1 ratio (one CBD gene from mom, one THC from dad). Then I bred a male and a female from that first generation and applied the alkaline alcohol trick to determine which had CBD. At that point I believed that if I bred a CBD+ male with a CBD+ female I had a good chance of getting some CBD-only seeds.
So I planted 4 of these seeds and all of them happened to be female*. Of these, three tested positive for CBD.
So far, so good! I could assume that half of these three would be 1:1 and half would be all CBD. Well, I "tested" my favorite one, which seemed to get me the least high. The lab came back with 7.7 CBD and 4.3 THC (I cut it off 3-4 weeks into flower). That's about 9/5. This doesn't follow with the neat ratio framework that I imagined.
Does anyone know how to explain this?
I am wondering if I have a tri or tetraploid plant?
*The fact that I got 4/4 females makes me wonder if maybe the mother hermmed and pollinated herself.
Thanks for reading, and I look forward to nerding out.
https://www.genetics.org/content/163/1/335
2) https://www.researchgate.net/figure/Chemotypes-and-CBD-THC-ratios-found-within-each-of-the-accessions-tested_tbl1_225148108 It looks like the ratios aren't as cut and dry as I thought. I don't know how the first link would explain this second one, but here is a list of CBD:THC ratios that have been tested.
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