Grain to grain transfer....pros and con?

MidnightXTRCTS

Active Member
Hello,
I've been reading the various ways to keep the life cycle of the mushroom rolling. It seems like spore prints, liquid solutions, cloning, and other similar teks are popular, tried, and true. However, as a newbie I am wondering if it is possible to use a jar that's 100% colonized to start other jars by using little pieces from the spawn? For example, I make 6 pieces from one fully developed rice cake, and put a piece into a new sterilized jar. Shouldn't the mycelium keep growing and developing and eventually colonized the jar? Please try to respond without laughing your ass off if this is a lame idea...or at least laugh after explaining why it would or wouldn't work. Thanks...
 

Gmz

Well-Known Member
It's totally possible, came across this video a while ago when I was learning that might help you.

 

MidnightXTRCTS

Active Member
Holy crap that is a great video. Thank you.
I really appreciate it. It's what I was thinking of exactly!
The level of precision with each movement and attention to detail and sterility makes me think this guy has done this a few hundred times. Quick but not hurried.
Is that squirt bottle alcohol or a diluted bleach solution?
 

butterbudface

Well-Known Member
You can grain to grain it. If your problem is that you don't have any culture, drop me a pm and i'll send you a swab.

I sterilize 15 spoons. warped in tinfoil for 20 mins at 15 psi. Take my fully colonized jar and give it a good shake. spoon out a lil bit in all the other jars and kaboom.

Best way to become self sustainable is to grow your cultures out on agar.

It is not difficult. Don't be scared to get into agar work, it's really easy. People say you need a FH but I do all my work in a crappy SAB and don't get contams ever. Ok maybe like one jar out of every 40 is a bad one.
 

DaSprout

Well-Known Member
You can grain to grain it....

I sterilize 15 spoons. warped in tinfoil for 20 mins at 15 psi. Take my fully colonized jar and give it a good shake. spoon out a lil bit in all the other jars and kaboom...
Exactly. As long as your at least decent in keeping things sterile (clean) it's really easy. The hardest part is the extra wait time. Cuz your like "Can't wait!".

Good luck.
 

Gmz

Well-Known Member

MidnightXTRCTS

Active Member
You can grain to grain it. If your problem is that you don't have any culture, drop me a pm and i'll send you a swab.

I sterilize 15 spoons. warped in tinfoil for 20 mins at 15 psi. Take my fully colonized jar and give it a good shake. spoon out a lil bit in all the other jars and kaboom.

Best way to become self sustainable is to grow your cultures out on agar.

It is not difficult. Don't be scared to get into agar work, it's really easy. People say you need a FH but I do all my work in a crappy SAB and don't get contams ever. Ok maybe like one jar out of every 40 is a bad one.
Thanks for the culture offer, I have some syringes of B+ and Golden Teacher ready to go. If I get proficient would love to swap spores in future.
Thanks for the encouragement, last time I tried growing was 22 years ago and I was a dumb teenager using my moms pressure cooker, using homemade potato dextrose agar, and spores ordered from Szygy! Needless to say total fail. I remember the agar and Petri dishes causing lots of condensation on the top lid, and getting a variety of mold and bacteria along with a little mycelium. It's was easier to go to Shakedown Street and get a bag of shrooms! 20 years later growing is a relaxing hobby... Wether it's tomatoes in the garden, pot in the grow room, or mycelium in a jar, it's relaxing to get away from the world for a minute or two.
Anyways, Rollitup is always very helpful and has never disappointed with members that are willing to share knowledge.
Thanks...
 

butterbudface

Well-Known Member
Thanks for the culture offer, I have some syringes of B+ and Golden Teacher ready to go. If I get proficient would love to swap spores in future.
Thanks for the encouragement, last time I tried growing was 22 years ago and I was a dumb teenager using my moms pressure cooker, using homemade potato dextrose agar, and spores ordered from Szygy! Needless to say total fail. I remember the agar and Petri dishes causing lots of condensation on the top lid, and getting a variety of mold and bacteria along with a little mycelium. It's was easier to go to Shakedown Street and get a bag of shrooms! 20 years later growing is a relaxing hobby... Wether it's tomatoes in the garden, pot in the grow room, or mycelium in a jar, it's relaxing to get away from the world for a minute or two.
Anyways, Rollitup is always very helpful and has never disappointed with members that are willing to share knowledge.
Thanks...
i must agree with the hobby is relaxing and it teaches you patience.

Try again bru it's not that hard. I place all my petris on top of each other with a mug with boiling water in on top of the top dish. Hope this makes sense. Its the difference in temp inside and outside the petri causing the condensation.

Pour some agar again. If your first plate is bacterial then just pour some more and transfer away from mold and bacteria. Thus cleaning up the plate.
 

MidnightXTRCTS

Active Member
i must agree with the hobby is relaxing and it teaches you patience.

Try again bru it's not that hard. I place all my petris on top of each other with a mug with boiling water in on top of the top dish. Hope this makes sense. Its the difference in temp inside and outside the petri causing the condensation.

Pour some agar again. If your first plate is bacterial then just pour some more and transfer away from mold and bacteria. Thus cleaning up the plate.
Totally understand. Thanks for explanation. There was no internet before when I tried. Stacking the petri dishes and a hot mug. I love it. That little book "Psilocybin, Magic Mushroom Grower's Guide" was all I had back then! Along with "Anarchist Cookbook", and "Poor Man's James Bond", those were my only resources to underground knowledge. Actually had to go to a bookstore. I'm probably dating myself... to the 1980's!
Now the world's knowledge is at our finger tips, just have to reach out and get it. Crazy.
Thanks for tip.
 
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