Bulk monotub shroom grow.
- Thread starter estesj
- Start date
ndangerspecimen101
Well-Known Member
A love tapping as I call it?
If you don't mind me asking Kushmasta how did you go about doing the liquid culture...
What items do you need and what's the range of difficulty?
If you don't mind me asking Kushmasta how did you go about doing the liquid culture...
What items do you need and what's the range of difficulty?
Sustainable420
Active Member
So, do you have to buy new syringes and spores every time you want to grow? Inquiring minds...
jfgordon1
Well-Known Member
Nope, you can clone
ANC
Well-Known Member
Easy liquid cultre
1 level tablespoon of spend (used) coffe grounds.
1 level teaspoon of sugar (brown preferably)
1 cup of boiled water...
Mix and let stand for 30 minutes.
Filter through coffee filter to remove particles. Add 2 more cups of boiled water.
Put in jar with some broken glass pieces. jar lid needs two holes sealed with silicon.
when you eventualy take stuff out of the jar you use two syringes, one in each "port".
One syringe is packed with cotton wool, air would pass through this to fill the vacuum as you suck liquid out through the other syringe, keeping airbased contaminants out.
Presure cook for 45 minutes... let cool and and put something in...
And no, you don;t need to buy new stuff... you can make clone cultres as explained above with the LC. or you can take spore prints and make your own syringes... Clones are definately the way to go, as they perform much better, and give nice even flush canopies.
1 level tablespoon of spend (used) coffe grounds.
1 level teaspoon of sugar (brown preferably)
1 cup of boiled water...
Mix and let stand for 30 minutes.
Filter through coffee filter to remove particles. Add 2 more cups of boiled water.
Put in jar with some broken glass pieces. jar lid needs two holes sealed with silicon.
when you eventualy take stuff out of the jar you use two syringes, one in each "port".
One syringe is packed with cotton wool, air would pass through this to fill the vacuum as you suck liquid out through the other syringe, keeping airbased contaminants out.
Presure cook for 45 minutes... let cool and and put something in...
And no, you don;t need to buy new stuff... you can make clone cultres as explained above with the LC. or you can take spore prints and make your own syringes... Clones are definately the way to go, as they perform much better, and give nice even flush canopies.
kushmasta88
Well-Known Member
For my liquid culture I used a half pint mason jar. You want to have a prepared solution ratio of 3-4% honey/Karo corn syrup to 100% water. What you do is measure 100 ml of water, add this into the half pint jar. Next add 3 to 4 ml of honey or karo along with a couple of marbles to agitate the growing mycelium in the future( VERY IMPORTANT STEP). Get some tyvek envelopes from your local post office and cut out 4''x4'' inch squares. Put these squares over the jar mouth, then put down your lid with two holes drilled into it, and finally screw the cap on. The tyvek serves as a barrier for contaminants and you can still poke a needle thru it. Cover one hole with silicone sealant which will reseal during needlework, and leave the other hole alone for air transport. People generally take more precaution with sterility of the holes but I say what the hell.
All you gotta do then is boil the jars sitting on a cloth for half an hour to an hour steady boil. Or be smart and pressure cook them sonnabitches. Just don't forget to leave the jar screwed loosely during sterilizing or you will indeed cause a vacuum pressure. Innoculate with 1-2cc's of spores, agitate with marbles everyday and you will have an advanced culture in a week or so if ur conditions were good. Hope this helps
All you gotta do then is boil the jars sitting on a cloth for half an hour to an hour steady boil. Or be smart and pressure cook them sonnabitches. Just don't forget to leave the jar screwed loosely during sterilizing or you will indeed cause a vacuum pressure. Innoculate with 1-2cc's of spores, agitate with marbles everyday and you will have an advanced culture in a week or so if ur conditions were good. Hope this helps
daisy2687
Well-Known Member
I ventured down here and decided to go hunting for those spore prints I took a while back... I think I THREW THEM AWAY WTFFFF... And my LC failed back then too.. Tried doing it with 'mycelium water' but the mycelium never grew again /shrug...
Definately going to clone on agar next time.
Anyone have some prints or syringes laying around? Trade maybe?
I like to run 4 66qt tubs... After 3 flushes usually get 3-4oz per tub... Summer time coming up..
Definately going to clone on agar next time.
Anyone have some prints or syringes laying around? Trade maybe?
I like to run 4 66qt tubs... After 3 flushes usually get 3-4oz per tub... Summer time coming up..
daisy2687
Well-Known Member
Actually I was thinking back and it failed simply cause I was trying something difficult. I wasnt doing any traditional LC methods. Essentially I was trying to follow a tek on how to clone using a piece of fresh fruit put in to LC liquid. It never worked. Never grew contams or anything but something I did wrong there.
Not too many people got it to work anyway but it does work for some.
I was just doing G2G transfer, also took spore prints but lost em... Now I gotta make some friends on the shroomery for a free print or order some up
Not too many people got it to work anyway but it does work for some.
I was just doing G2G transfer, also took spore prints but lost em... Now I gotta make some friends on the shroomery for a free print or order some up
ndangerspecimen101
Well-Known Member
I essentially want to learn on how to make a spore print!
But this cloning process sounds like a heck of a procedure...and its on my to do list in the near future!
So many methods, so little time
But this cloning process sounds like a heck of a procedure...and its on my to do list in the near future!
So many methods, so little time
daisy2687
Well-Known Member
Spore print super easy. Let the cap open all the way, harvest just before or right after spores start dropping.I essentially want to learn on how to make a spore print!
But this cloning process sounds like a heck of a procedure...and its on my to do list in the near future!
So many methods, so little time
Sterelize everything used below with rubbing alcohol (hand included) (also allow alcohol to evap)
Stick a thumb tack in the top middle of the cap. Cut cap off very close to gills. Place on tin foil twice the size of the cap. Place jar over top of tin foil and cap. Place in warm (75-85) area. Wait 24h. Cap should have shrunk a little and left very dark spore print with billions of spores
. Remove cap and consume or dry.Fold over foil, place in plastic bag and place in dark dry place.
Enough spores for at least 10 syringes.
ndangerspecimen101
Well-Known Member
Yikes, what a simplistic way you put that!Spore print super easy. Let the cap open all the way, harvest just before or right after spores start dropping.
Sterelize everything used below with rubbing alcohol (hand included) (also allow alcohol to evap)
Stick a thumb tack in the top middle of the cap. Cut cap off very close to gills. Place on tin foil twice the size of the cap. Place jar over top of tin foil and cap. Place in warm (75-85) area. Wait 24h. Cap should have shrunk a little and left very dark spore print with billions of spores
Fold over foil, place in plastic bag and place in dark dry place.
Enough spores for at least 10 syringes.
Cant' wait for the big harvest!
ANC
Well-Known Member
lol now combine that with the liquid culture, take an LC jar make a cap and silicone a paperclip through the lid that has the inside shaped like a hook.... attach mushroom cap, put back on jar with sterilised LC in glovebox... and spores are collected in liquid culture... or alternatively distilled water can be used to collect spores for makeing new syringes.
ANC
Well-Known Member
Takeing spore prints and being able to make your own syringes allows you to swap strains with someone far away. And also to store them for the medium term.
Also if the companies who provide the syringes gets closed down, you are still independant, also no traces of credit card billing etc.
I have never bought a single premade syringe in my life. makeing one costs cents... makeing a print costs only a piece of tinfoil.
Idealy you only want to work with spores once per strain, then isolate and clone the best fruits as this will allow you bigger more even flushes of a colony of fungi that is not competeing with itself.
Also if the companies who provide the syringes gets closed down, you are still independant, also no traces of credit card billing etc.
I have never bought a single premade syringe in my life. makeing one costs cents... makeing a print costs only a piece of tinfoil.
Idealy you only want to work with spores once per strain, then isolate and clone the best fruits as this will allow you bigger more even flushes of a colony of fungi that is not competeing with itself.
ndangerspecimen101
Well-Known Member
Cost effectiveness... now that's what ANC has thought me
