Homemade Lactobacillus Serum

sworth

Well-Known Member
I just finished making my first lactobacillus serum the other day. Each stage took a week. I've ended up with 750ml/ 1.3 pints of serum after mixing with molasses.
Good timing, for me, finding out lactobacillus even existed, as I'm busying myself with my first organic soil mix
Following this thread to watch the pros and cons of teas etc... I'd better roll a fat one then I guess :bigjoint:
 

Sunny Organics

Well-Known Member
so the mixture i ended up with was filtered out with some coffee filters 2x until i got my yellowish water. the reason it was white it was because the water was mixed with some kind of cream or yogurt lol. after it sat for a while, i saw how the yellow water seperated from the cream, so i bought some coffee filters to strain it...

here's my result - http://puu.sh/ibOij/df6841dd3c.jpg So the ratios are 12oz of molasses per 12 oz of the yellow water.

I'll try it out friday on their next tea watering, stay lifted my friends thanks for the recipe!!! :bigjoint::peace::leaf:
 

nudger36

Well-Known Member
Great thread :) I made some of this but my curd is in the bottom with the light yellowy kind of serum at the top, just wondered if I have to wait for the curd to rise before separating please? did do a big batch so maybe just taking longer. Look forward to your reply :)
 

sworth

Well-Known Member
I think separation is the key...
(10 months after I made my 750ml/ 1.3 pints of serum I still have loads left (:)
 

shaft09

Well-Known Member
MjMamasan, I did it! But I forgot to take pics of the first batch. Took about two weeks total, but I let the curds go a few days longer than needed.

This morning I strained and mixed with molasses. Got it in the fridge right now until I need it. As you can see in the pic, got one more batch going, should be ready by Friday.

Gonna give it to everything I have growing. Really want to use it on the veggie garden to see if it perks up 1 mater plant that is looking not as good as as the others. Hope it does me good!image.jpegimage.jpeg
 

Attachments

iHearAll

Well-Known Member
Hi everyone. I've been working on making my own lacto serum, similar to the expensive EM1 product. Sorry if this info has already been shared here.

First, take a 1/4 cup of rice and shake it in 1 cup of warm water until the water gets cloudy.
View attachment 3413567

This is harvesting the starch from the rice that will attract beneficial bacteria from the air into the water. Strain and keep the rice rinse water, and discard the rice. You can add the used rice to your compost pile so it doesn't go to waste. Microbes and whatnot love rice.

Keep the starchy water in a jar under your sink for a week or two to attract the bacteria we are seeking. After the wait period I drain off and keep the top 95 percent of the water and leave the thick white sludge that has formed at the bottom. We don't need that part.

Now, for every one part rice water, add 10 parts milk. So for 1 cup water it takes 10 cups of milk. Any type of milk is fine. I just filled a mason jar to the top and called that good enough. It doesn't have to be an exact science. Again, let this mixture sit. This time a few days should be all it takes for the milk to curdle. The milk solids will rise to the top and look like cottage cheese.
View attachment 3413570

Strain out the curds and keep the yellow liquid that remains. Now mix the yellow liquid 50/50 with molasses and you have your finished product that can be stored for up to 6 months.

I use 2-3 tbs per gallon for foliar and 1/3 cup per gallon for soil drench.

The cheese curds can be fed to dogs or added to the compost. My dogs love it and it's great for their digestive health. It's a probiotic for plants and pets.
EM should only cost $1-2 per bottle
heres why!


Cool thing about lab grown EM1 is it's diverse yet constistently probiotic and can be cultured into more volume. Lactoserum focuses on native cultures and exclusively lactobacilli.


To make an extended bottle of EM1 or as I have learned to call it EMe, you need:

nonchorinated water,

agricultural grade molasses, this is about 10-20 a gallon from a local supplier and I imagine eBay would have some decent products

liter size plastic bottle,

tablespoon (15mL),

funnel,

sharpie

and a bottle of EM1 from DR HIGA @ TERAGANIX don’t buy dormant bacteria from anywhere other than the lab their cultured at.

Procedure:

Measure out two tablespoons of water and pour it into the plastic bottle, mark this level with the sharpie. This is a 30mL mark for the molasses.

Pour out the water and pour in molasses up to this sharpie line.

Add ¾ liter of water or enough to dissolve the molasses by spinning or flipping, but not shaking. Shaking will produce a lot of foam and will have to settle. Just a hassle.

Next add two tablespoons of EM1 to the bottle

Fill the rest of the bottle but leave a little air space at the top. The bottle will produce pressure and need to be burped once or twice throughout the first week. For larger batches, a carboy can be used. These usually have airlocks which will prevent a pressure build up.

So, the bottle need to ferment with the lid tight for a week. Keep it out of the sun or you’ll wake up the photosynthetics. After a week has gone by, the bottle can be considered EMe or ‘’EM extended”. You can drink it straight, make bokashi, use it in your garden, control septic tank sludge, control pet odors, etc etcetcetcetcetc.

It should be diluted in water @1:100 ratio by volume with an equal amount of molasses. For instance, If you added 10mL of EMe then you will need 10 mL of molasses. The sugar is the food but white sugar is a poor diet and won’t ferment correctly.

Extra:

EM1 should be used to make EMe. EMe should not be used to make more EMe, it’ll only be lactobacillus being cultured, which is OK but not as effective as EM1 or EMe.

There are a million fertilizers you can make with EMe. Fermented fruits, weeds, adding a science to biodynamics, use in ACT.





Here's EM1:
Specifications
ACTIVE INGREDIENTS*:
Microorganisms: 1 million colony forming units/cc (units/ml), 1%: Lactic Acid Bacteria, Yeast, and Photosynthetic Bacteria

INACTIVE INGREDIENTS:
96% Water and 3% Molasses

*Not all microbes are listed on the actual label.



Just for your own sanitation's sake, the rice wash should be used up in a week. The bacteria will shift powers to a less active culture and begin to spoil. Starting with a lab grown culture is much healthier. You'd need a compound microscope to faithfully say you're culturing only probiotics. A liter of lab grown EM1 can make 33.3 high quality liters of EMextended.
2016Price of EM1 23$ without shipping. So 30$ with shipping
30÷33=91 cents per new bottle


A gallon of agricultural grade molasses is 5-20$ store to store. And will feed 135 liter size bottles of EMextended.


So, lets pretend like molasses was $20.
20÷135=15cents per bottle

$1.06 per liter of lab quality EMe (EM extended).
 

iHearAll

Well-Known Member
Hi everyone. I've been working on making my own lacto serum, similar to the expensive EM1 product. Sorry if this info has already been shared here.

First, take a 1/4 cup of rice and shake it in 1 cup of warm water until the water gets cloudy.
View attachment 3413567

This is harvesting the starch from the rice that will attract beneficial bacteria from the air into the water. Strain and keep the rice rinse water, and discard the rice. You can add the used rice to your compost pile so it doesn't go to waste. Microbes and whatnot love rice.

Keep the starchy water in a jar under your sink for a week or two to attract the bacteria we are seeking. After the wait period I drain off and keep the top 95 percent of the water and leave the thick white sludge that has formed at the bottom. We don't need that part.

Now, for every one part rice water, add 10 parts milk. So for 1 cup water it takes 10 cups of milk. Any type of milk is fine. I just filled a mason jar to the top and called that good enough. It doesn't have to be an exact science. Again, let this mixture sit. This time a few days should be all it takes for the milk to curdle. The milk solids will rise to the top and look like cottage cheese.
View attachment 3413570

Strain out the curds and keep the yellow liquid that remains. Now mix the yellow liquid 50/50 with molasses and you have your finished product that can be stored for up to 6 months.

I use 2-3 tbs per gallon for foliar and 1/3 cup per gallon for soil drench.

The cheese curds can be fed to dogs or added to the compost. My dogs love it and it's great for their digestive health. It's a probiotic for plants and pets.

more diverse home culture for cheaper than lactoserum or EMe

How-to-Grow-a-Scoby-DSC_0787.jpg
SCOBY mushroom or better known as kombucha. This is typically a carbonated beverage that houses billions of microorganisms. I encourage you to follow a drinkable kombucha recipe to become familiar with this organism.

For this bit, I'm under the impression that many cannabis growers use lactoserum which as the name suggests cultivates lactobacilli.

This gelatinous colony of bacteria will help you culture the following strains,


  • Acetobacter [2] is an aerobic (requiring oxygen) bacteria strain that produces acetic acid and gluconic acid. It is always found in kombucha. Acetobacter strains also build the scoby mushroom. Acetobacter xylinoides and acetobacter ketogenum are two strains that you might find in kombucha.
  • Saccharomyces [2] includes a number of yeast strains that produce alcohol and are the most common types of yeast found in kombucha. They can be aerobic or anaerobic (requires an oxygen-free environment). They include Saccharomycodes ludwigii, Saccharomycodes apiculatus, Schizosaccharomyces pombe, Zygosaccharomyes, and Saccharomyces cerevisiae.
  • Brettanomyces [2] is another type of yeast strain, either aerobic or anaerobic, that are commonly found in kombucha and produce alcohol or acetic acid.
  • Lactobacillus [2]: A type of aerobic bacteria that is sometimes, but not always, found in kombucha. It produces lactic acid and slime.
  • Pediococcus [2]: These anaerobic bacteria produce lactic acid and slime. They are sometimes, but not always, found in kombucha.
  • Gluconacetobacter kombuchae [2] is an anaerobic bacteria that is unique to kombucha. It feeds on nitrogen that is found in tea and produces acetic acid and gluconic acid, as well as building the scoby.
  • Zygosaccharomyceskombuchaensis [3] is a yeast strain that is unique to kombucha. It produces alcohol and carbonation as well as contributing to the mushroom body
http://www.culturesforhealth.com/learn/kombucha/kombucha-bacteria-yeast/

This will be a slightly more complicated brew of bacteria but can be used to control powdery mildew as reported by some, has a symbiotic relationship with your inferior human digestive track, and will help the well-being of your environment. Can be diluted and sprayed similar to every other inoculation mentioned previously, at least 1:100-250 by volume. For PM i suggest starting there and increasing. Spray at night and dilute more during flower.
 

hyroot

Well-Known Member

shaft09

Well-Known Member
There's some wrong info in this thread. read this below. This is what I follow. it's more detailed than the unconventional farmer recipe.


https://docs.google.com/document/d/1cxlUTCpm6NCKGWiMVpmA5YlO6J2kCu5g6QaNXfgjauo/mobilebasic?pli=1


I don't know who Roberto is or why he would put his number up there. this is all over the probiotic farmers alliance group in Facebook. The owner of gro kashi is one of the mods of the group
Think I'll stick with what I have. Thanks for the detailed info.
 

MjMama

Well-Known Member
I've been making it this way for a few years now with good results. Never had any complaints from others who used this method. But if you have your own way to make it, more power to you.
 

iHearAll

Well-Known Member
Do it all!! I make lactoserum but haha i pour it in my septic tank and on compost by the gallon. I add EMe to it to boost it along. I make so many ferments.
 

iHearAll

Well-Known Member

MjMama

Well-Known Member
more diverse home culture for cheaper than lactoserum or EMe

View attachment 3699436
SCOBY mushroom or better known as kombucha. This is typically a carbonated beverage that houses billions of microorganisms. I encourage you to follow a drinkable kombucha recipe to become familiar with this organism.

For this bit, I'm under the impression that many cannabis growers use lactoserum which as the name suggests cultivates lactobacilli.

This gelatinous colony of bacteria will help you culture the following strains,


  • Acetobacter [2] is an aerobic (requiring oxygen) bacteria strain that produces acetic acid and gluconic acid. It is always found in kombucha. Acetobacter strains also build the scoby mushroom. Acetobacter xylinoides and acetobacter ketogenum are two strains that you might find in kombucha.
  • Saccharomyces [2] includes a number of yeast strains that produce alcohol and are the most common types of yeast found in kombucha. They can be aerobic or anaerobic (requires an oxygen-free environment). They include Saccharomycodes ludwigii, Saccharomycodes apiculatus, Schizosaccharomyces pombe, Zygosaccharomyes, and Saccharomyces cerevisiae.
  • Brettanomyces [2] is another type of yeast strain, either aerobic or anaerobic, that are commonly found in kombucha and produce alcohol or acetic acid.
  • Lactobacillus [2]: A type of aerobic bacteria that is sometimes, but not always, found in kombucha. It produces lactic acid and slime.
  • Pediococcus [2]: These anaerobic bacteria produce lactic acid and slime. They are sometimes, but not always, found in kombucha.
  • Gluconacetobacter kombuchae [2] is an anaerobic bacteria that is unique to kombucha. It feeds on nitrogen that is found in tea and produces acetic acid and gluconic acid, as well as building the scoby.
  • Zygosaccharomyceskombuchaensis [3] is a yeast strain that is unique to kombucha. It produces alcohol and carbonation as well as contributing to the mushroom body
http://www.culturesforhealth.com/learn/kombucha/kombucha-bacteria-yeast/

This will be a slightly more complicated brew of bacteria but can be used to control powdery mildew as reported by some, has a symbiotic relationship with your inferior human digestive track, and will help the well-being of your environment. Can be diluted and sprayed similar to every other inoculation mentioned previously, at least 1:100-250 by volume. For PM i suggest starting there and increasing. Spray at night and dilute more during flower.
I'm sorry but you sound like a paid rep pushing products, not someone trying to help make products at home for cheaper.
 

iHearAll

Well-Known Member
I'm sorry but you sound like a paid rep pushing products, not someone trying to help make products at home for cheaper.
Oh thanks. ...But anyway...
Kombucha is a naturally occuring culture of bacteria i can just feed sugar, a small amount of the previous batch, and flavor with tea if i want to drink it. Helps control PM. God's the only one getting paid there. And he doesn't need our mortal money. EM is just cheap if I culture it from the bottle. I'm just formally educated with these techniques. I trained formally in a 3rd world Philippines in sustainable agriculture, this is pretty much who wrote the book on kimchi farming. The program and teachers focused on proving the benefits of probiotics and probiotic/high brix fruits and vegetables to feed the foster children on the farm and train the island people as well as from countries all over the planet.

So, naturally i sound like a rep because I know my KNF inside and out, my math, and reasons why you should consider using lab quality materials. Lactoserum has nothing preventing pathogens from joining the ride into your bokashi, extracts, and soil.

I should have said i like your techniques first. Then said "here's what i do. It works too! Cheers"

Problem with only saying that is many people want to hear why someone can claim something superior. Credibility and such. Fuck i typed too much
 
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