Innoculating Grain Spawn bags, How many CC's to use.

I have just finished my first grow of mushies, doing pf tek. I am about to start back up again and am NOT going with cakes i am going to take a wack at bulk. I have spawn bags filled with 2lbs of grain in each. How many cc's of spores will I need per bag?

I also took a couple spore prints from my last grow (Cambodians) and will more than likely use them. I have an idea of how to make the spore water but how many cc's can I get from one spore print? I feel like this number cannot be exact as some of my prints are larger than others. Is it just something you eye up.


Thanks guys for your insight

SirLancelot said:

I have just finished my first grow of mushies, doing pf tek. I am about to start back up again and am NOT going with cakes i am going to take a wack at bulk. I have spawn bags filled with 2lbs of grain in each. How many cc's of spores will I need per bag?

I also took a couple spore prints from my last grow (Cambodians) and will more than likely use them. I have an idea of how to make the spore water but how many cc's can I get from one spore print? I feel like this number cannot be exact as some of my prints are larger than others. Is it just something you eye up.


Thanks guys for your insight

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What Bulk method are you using?

One dark, nice size print can be used, in theory, to make several syringes, but I find it easier just to use the whole thing. Inject your sterile water, into the clean bag containing the clean print and suck it back up. No mess, no hassle, and everything stays sterile if you are careful. I don't do bags, but 1cc is more than enough for a qt jar. If you're taking lots of prints, then spores and spore solution is pretty much free so no need to skimp imo.

sonar said:

One dark, nice size print can be used, in theory, to make several syringes, but I find it easier just to use the whole thing. Inject your sterile water, into the clean bag containing the clean print and suck it back up. No mess, no hassle, and everything stays sterile if you are careful. I don't do bags, but 1cc is more than enough for a qt jar. If you're taking lots of prints, then spores and spore solution is pretty much free so no need to skimp imo.

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For some reason I feel like I read that 140cc is necessary for a bulk bag but I don't know how big that was. Yea I took my prints inside ziploc bags so like you said All I gotta do is add water and suck it up. I did have a couple of my prints fall off of a shelf and crumplin into pieces, this wouldn't cause any issues would it? I mean the bag is still sealed and all. Yea I was kind of thinking make like 10 or more 10cc syrnges from a couple of my prints.

The Cryptkeeper said:

What Bulk method are you using?

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I am just doing rye grain bags and going to put em in trays and case em. I then put the trays in my semi-automated aquarium. Atleast I believe this is what I do. lol

I'd take the grain bag and spawn it too h-shit/verm mix...70% shit 30% verm...pasturise enough shit mix to load your tray (I use kitty litter trays) 3 inches deep...any deeper invites contams...I use 3 pints of grain spawn to one kitty litter tray...keep enough shit-mix to give the final non-inoculated topping of 1/2 inch....cover the tray with tinfoil and punch some tiny holes( I use a specimen pin from a microscope kit ) randomly in the foil for GE...set tray in a quiet place...colonize/fruiting temps means not alot to me personally...the greeny meany loves it around 80 degrees...I go around 70-75...this simply means slower colonization...an extra week maybe...after a week take a quick peek from a corner of the tray...when the top is totally white, leave it covered up for 5-7 days to consolidate the myc's hold on the sub...then take the foil off and toss it in yer fruiting chamber...I use 2 mini greenhouses equipped with ultrasonic misters and temp/hygrometers...I manually fan the houses 3-4 times a day...try to keep humidity around 90-99%...I never achieve these measures but find I still get good flushes at 80% humidity...sorry for the long post...hope it helps someone...))

Hell yea man that sounds legit. Like I said Im new to this and still kinda getting a grasp. I do know Im not going back to cakes.. their just not for me. For some reason I was under the impression I could just use the grain. But your method sounds like it's a much bigger yielder with the h/shit verm mix! My only problem is my lil aquarium is only a 10 gal so I may be able to fit one tray. I am still curious though to how much spores I should use to innoculate? how much do you use per pint or however you do it?

when I use MS its about 2ml per pint....LC is 1ml per quart

MS? LC? sorry man is MS spore print? and LC liquid culture?

SirLancelot said:

MS? LC? sorry man is MS spore print? and LC liquid culture?

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lol Great link.
http://www.shroomery.org/9423/Mycological-and-OMC-abbreviations

SirLancelot said:

MS? LC? sorry man is MS spore print? and LC liquid culture?

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MS is Multi Spore.....LC is Liquid Culture...

Hey guys could I get your expertise one last time. Do you think it is necessary for someone such as myself who is new to this to go and make a liquid culture to innoculate my bags? or would it just be easier to make a spore syringe from my print? Im only worried with regular spore syringe it's going to take multiple injections. Of course I have no idea which is why I'm asking. So the LC pretty much is just a jump start on getting the spores started and you take your LC and just inject your bag with it?

The Cryptkeeper said:

What Bulk method are you using?

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yea I was mistaken I am doing organic rye berries spawn bags.

SirLancelot said:

Hey guys could I get your expertise one last time. Do you think it is necessary for someone such as myself who is new to this to go and make a liquid culture to innoculate my bags? or would it just be easier to make a spore syringe from my print? Im only worried with regular spore syringe it's going to take multiple injections. Of course I have no idea which is why I'm asking. So the LC pretty much is just a jump start on getting the spores started and you take your LC and just inject your bag with it?

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I don't make LC's but I myself am tempted to just inoculate my Monotub with some of the various spore syringes I got this Halloween. Is it necessary? No.

lol no but sounds fun!

That would be pretty cool to have different lil types of mushies growing. I am tempted to grab another strain before I start this next one to have two different types.

I don't know but I can't image there being much of a difference in the differenty types of psylicin mushrooms. I mean psilicybin is psylicybin please correct me if Im wrong.

I started using lc a few months ago and never looked back. So much more efficient than using spore water. Cuts colonization time down in quart jars of grains and also pf jars by at least a week. Saves on spore solution too if you aren't into spore printing.

What you mean cryptkeeper you just want to shoot up a tub of coir or hpoo with straigh spore solution? I think it would work lc in a highly clean environment, but out back in the garage or down the basement it would probably contam long before it fully colonized.

so basically liquid culture is just a place where you can inject some spore prints (syringe) and they are able to survive and grow in the LC thus shaving time from using regualr spore syringes. are there like ALOT more spores in say a 10cc syringe of LC compared to regualr spore syringe? or is it there just at different stages of growth?

take a packet of raw sugar...SITR (Sugar In The Raw) and add it to a 2/3rds full of h2o in a 1/2 pint mason jar preped with a silicon port and a port for GE (Gas Exchange )....PC for 20 mins at 10 psi...remove from heat and allow pressure to bleed off naturally...when cool inoculate thru silicone port and swirl every other day...once you have a good amount of myc just store in the fridge...just how I prepare my LC...lots of ways out there...hth

sonar said:

I started using lc a few months ago and never looked back. So much more efficient than using spore water. Cuts colonization time down in quart jars of grains and also pf jars by at least a week. Saves on spore solution too if you aren't into spore printing.

What you mean cryptkeeper you just want to shoot up a tub of coir or hpoo with straigh spore solution? I think it would work lc in a highly clean environment, but out back in the garage or down the basement it would probably contam long before it fully colonized.

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LOL Shoot up jars of WBS and then do the tub up like in here,
https://www.rollitup.org/hallucinatory-substances/395504-bulk-mono-tek-pictorial.html#post5096821

SirLancelot said:

so basically liquid culture is just a place where you can inject some spore prints (syringe) and they are able to survive and grow in the LC thus shaving time from using regualr spore syringes. are there like ALOT more spores in say a 10cc syringe of LC compared to regualr spore syringe? or is it there just at different stages of growth?

Click to expand...

Different stages of growth. The idea is that the spores germinate creating a mycelium mass suspending in a weak (2-4%) sugar solution. I use corn syrup. It's cheap, effective, and a lot of people have it sitting in the cupboard anyway. Honey also works. I bought some syringe filters and use them as my airport. When you go to suck some out, you need a way to let clean air in to replace the volume of liquid you are removing.

You can get away with using as little as a single drop of spore solution - if the solution is dense. All you are attempting to do is get sufficient spores into the bag so that at least two of the spores are sexualy compatible. I figure 1 ml is plenty regardless. Any time you lose starting so slowly is fine because you will be shaking the bag a day or so after you see white, and a day or so after you see white again, and a day or so after you see it again - which should have your whole bag colonized in about 10 days if your temperatures are right.