Sustainable Organics Garden

iHearAll

Well-Known Member
So we really don't know yet what triggers this?! Exciting! :D
Oh please let it be a combination of LED and totally microbial soil haha
I'll definitely keep an eye out, it's intriguing!

But do tell us more about this Tony, he is analysing these anthocyanins chemically then?!
from observation of purpling strains it is weather induced from temps dropping by 15F degrees. so like, 70F day to 55F night temp swing would induce purpling of many genetics. i would imagine the cyanins "bleed" into the trichomes and would make for a pretty picture with a strain that remains green but trichs soak up purples froooom the veins? yea idk... cold temps is a good place to start maybe someone will time lapse a microscopic view of some trichs going blue to give a better understanding of it's origins
 

NaturalFarmer

Well-Known Member
from observation of purpling strains it is weather induced from temps dropping by 15F degrees. so like, 70F day to 55F night temp swing would induce purpling of many genetics. i would imagine the cyanins "bleed" into the trichomes and would make for a pretty picture with a strain that remains green but trichs soak up purples froooom the veins? yea idk... cold temps is a good place to start maybe someone will time lapse a microscopic view of some trichs going blue to give a better understanding of it's origins
Have you tried ice water at the end of flower?
 

iHearAll

Well-Known Member

Folks love purple pot. But what causes this variation and what can be done to manipulate it further? This report from our special correspondent, Chewberto420, breaks it down.

What Makes Cannabis Purple?



The answer is Anthocyanins. The word Anthocyanin was created by a German botanist named Ludwig Marquart in 1835. The word "anthocyanin" is derived from two Greek words – anthos meaning Flower and Kyanos meaning blue, which translate to “Blue Flower.” Anthocyanins are water-soluble flavonoids which contain coloring pigment usually found in the epidermal layer of plants. The range of the color spectrum vary from blues and purples, to pinks and magentas depending on the pH. The anthocyanins are present within the vacuole of the cells in plants tissues, leaves, fruits, and flowers. The pigment can also travel up trichome stalks from the bract where the trichome is attached, and even sometimes within the trichome head itself. Anthocyanins are powerful pigments compounds, and cannabis only needs to produce small amounts of these chemicals to produce a strong color.





Why are Anthocyanins Present?



During a plant's life cycle, the leaves go through changes creating phytochemicals for various reasons which give plants their color, anthoxanthins (white/cream), carotenoids (yellow/orange), chlorophyll (green), lycopene (red), and cnthocyanin which make up the vibrant purple and blue color in plants. Each of these compounds play a unique roll in the nature of a plant's life, as well as its survival.

  1. Anthocyanins serve to protect photosynthetic tissues from stress brought on by different light intensities and spectrums. Essentially, the anthocyanin acts as a sunscreen for the leaves. (It should be noted that LED lights with sophisticated spectrums could aid in increased anthocyanin production.)
  2. Anthocyanin production is also thought to be a nectar guides for pollinators such as butterflies, hummingbirds, bees, as well as deter some insects from laying eggs on the foliage by tricking them into thinking the plant is unhealthy.
Anthocyanin Extraction from Cannabis

It is possible to extract anthocyanins with hash oil by applying a technique called “Rosin,” popularly known as “Rosin Tech."

Rosin Tech is the process of using steam vapor pressure to penetrate the cuticle/membrane of the trichome heads and trichome stalks on the flower/bud to release the essential oils containing the sensitive terpenes and cannabinoids within. This process can create a colorful hash oil if done early enough in the hang-dry process at harvest.

Typically, Rosin Tech is applied when the flowers have gone through a complete hang-dry process, and a majority of moisture has dissipated from the flowers. After the water content has been reduced drastically, the pH of the plant has increased, and the hash oil tends to be a golden/yellow. However, if we allow the flowers/buds to maintain their acidic pH early in the hang-dry process or right at harvest, the anthocyanins stay mobile and soluble and are extracted with the trichomes, essentially “dyeing’ the oil purple as it is forced away from the flower.

There is a short window of time in which one is able to extract these colors. As the plant dries, its pH starts to change making it impossible for the color to be extracted. The reason being that anthocyanins are highly unstable and extremely susceptible to degradation. Many factors can attribute to their degradation, such as temperatures, pH, light, and oxygen amongst other exposures.

The sensitivity of anthocyanins can be observed while dabbing the Anthocyanin dyed rosin, As the oil comes in contact with heat exposure, you’ll notice the oil return to the colorless carbinol base, and the colorful pigments in the hash oil will disappear immediately.

In Closing

The mysteries behind anthocyanins are relatively unknown. Can we preserve our health, food, and concentrates longer by incorporating these anthocyanins? Could there be more to add to the list of benefits? Anthocyanins are not present in cannabis in high enough amounts to be considered an active ingredient, but we may not have all the answers quite yet, but with all that we do know it would be within good reasoning to continue testing and expand research.

Don’t forget to eat your blueberries!

All photos by Chewberto420 except for trichome shots, photographed by Biergoggles



GROW
 

calliandra

Well-Known Member
Folks love purple pot. But what causes this variation and what can be done to manipulate it further? This report from our special correspondent, Chewberto420, breaks it down.

What Makes Cannabis Purple?



The answer is Anthocyanins. The word Anthocyanin was created by a German botanist named Ludwig Marquart in 1835. The word "anthocyanin" is derived from two Greek words – anthos meaning Flower and Kyanos meaning blue, which translate to “Blue Flower.” Anthocyanins are water-soluble flavonoids which contain coloring pigment usually found in the epidermal layer of plants. The range of the color spectrum vary from blues and purples, to pinks and magentas depending on the pH. The anthocyanins are present within the vacuole of the cells in plants tissues, leaves, fruits, and flowers. The pigment can also travel up trichome stalks from the bract where the trichome is attached, and even sometimes within the trichome head itself. Anthocyanins are powerful pigments compounds, and cannabis only needs to produce small amounts of these chemicals to produce a strong color.





Why are Anthocyanins Present?



During a plant's life cycle, the leaves go through changes creating phytochemicals for various reasons which give plants their color, anthoxanthins (white/cream), carotenoids (yellow/orange), chlorophyll (green), lycopene (red), and cnthocyanin which make up the vibrant purple and blue color in plants. Each of these compounds play a unique roll in the nature of a plant's life, as well as its survival.

  1. Anthocyanins serve to protect photosynthetic tissues from stress brought on by different light intensities and spectrums. Essentially, the anthocyanin acts as a sunscreen for the leaves. (It should be noted that LED lights with sophisticated spectrums could aid in increased anthocyanin production.)
  2. Anthocyanin production is also thought to be a nectar guides for pollinators such as butterflies, hummingbirds, bees, as well as deter some insects from laying eggs on the foliage by tricking them into thinking the plant is unhealthy.
Anthocyanin Extraction from Cannabis

It is possible to extract anthocyanins with hash oil by applying a technique called “Rosin,” popularly known as “Rosin Tech."

Rosin Tech is the process of using steam vapor pressure to penetrate the cuticle/membrane of the trichome heads and trichome stalks on the flower/bud to release the essential oils containing the sensitive terpenes and cannabinoids within. This process can create a colorful hash oil if done early enough in the hang-dry process at harvest.

Typically, Rosin Tech is applied when the flowers have gone through a complete hang-dry process, and a majority of moisture has dissipated from the flowers. After the water content has been reduced drastically, the pH of the plant has increased, and the hash oil tends to be a golden/yellow. However, if we allow the flowers/buds to maintain their acidic pH early in the hang-dry process or right at harvest, the anthocyanins stay mobile and soluble and are extracted with the trichomes, essentially “dyeing’ the oil purple as it is forced away from the flower.

There is a short window of time in which one is able to extract these colors. As the plant dries, its pH starts to change making it impossible for the color to be extracted. The reason being that anthocyanins are highly unstable and extremely susceptible to degradation. Many factors can attribute to their degradation, such as temperatures, pH, light, and oxygen amongst other exposures.

The sensitivity of anthocyanins can be observed while dabbing the Anthocyanin dyed rosin, As the oil comes in contact with heat exposure, you’ll notice the oil return to the colorless carbinol base, and the colorful pigments in the hash oil will disappear immediately.

In Closing

The mysteries behind anthocyanins are relatively unknown. Can we preserve our health, food, and concentrates longer by incorporating these anthocyanins? Could there be more to add to the list of benefits? Anthocyanins are not present in cannabis in high enough amounts to be considered an active ingredient, but we may not have all the answers quite yet, but with all that we do know it would be within good reasoning to continue testing and expand research.

Don’t forget to eat your blueberries!

All photos by Chewberto420 except for trichome shots, photographed by Biergoggles



GROW
Wow thanks for sharing that!
The way he mentions the relationship of pH on the color range that will be expressed, doesn't it remind you of hydrangeas too?! I went and checked, and apparently the hydrangea has a special anthocyanide, so the ones in cannabis could be different ones.
It was an interesting read anyway - and they do experiments to see how they can influence the colors chemically too - goes way over my head, but you witchy guys may find something in there :) http://www.americanscientist.org/issues/pub/2014/6/curious-chemistry-guides-hydrangea-colors/3
 

iHearAll

Well-Known Member
have ya seen that triple purple rhino rosin that is inkly purple almost black ?
simple heat pressing onto parchment paper kinda blows some of what he said
search IG #triplepurplerhino or #TPR
woah that is beautiful, i just looked that up.

i wish i took cuttngs of CC black kush this spring. it got mauled by outdoor mites and wasnt a happy cat but would have been great to play with indoors this time of year.
 

iHearAll

Well-Known Member
Wow thanks for sharing that!
The way he mentions the relationship of pH on the color range that will be expressed, doesn't it remind you of hydrangeas too?! I went and checked, and apparently the hydrangea has a special anthocyanide, so the ones in cannabis could be different ones.
It was an interesting read anyway - and they do experiments to see how they can influence the colors chemically too - goes way over my head, but you witchy guys may find something in there :) http://www.americanscientist.org/issues/pub/2014/6/curious-chemistry-guides-hydrangea-colors/3
although the article doesnt come out and directly suggest adding trace element/rare metals is important they suggest the use of them is helping form these blue hues. i imagine adding azomite or such can help boost up blues. i also noticed the elements discribed are also the elements that can be combine in specific ratios to make the semiconductors for these new exotic UV LEDs. ..fascinating.
 

calliandra

Well-Known Member
although the article doesnt come out and directly suggest adding trace element/rare metals is important they suggest the use of them is helping form these blue hues. i imagine adding azomite or such can help boost up blues. i also noticed the elements discribed are also the elements that can be combine in specific ratios to make the semiconductors for these new exotic UV LEDs. ..fascinating.
Ohwow!
While at first I was very unsure whether what has been researched regarding the hydrangea's colors would be at all applicable to cannabis, this does support the supposition that the mechanisms at work could becommon to all anthocyanides.
In reading around, I saw that it is often recommended to raise the soil acidity by mulching the hydrangeas with hi-N stuff (grass clippings, coffee grounds,...). Yes, it's not the pH in itself, but we have all the minerals we'll ever need in the soil, all we need are the right microbes to mine them out.
So if initiating composting directly next to the plant will make hydrangeas go red, and @Rasta Roy 's microherd is diverse and healthy, we may see those pink trich's in his big-ass pot garden soon eh :bigjoint:
 

iHearAll

Well-Known Member
Ohwow!
While at first I was very unsure whether what has been researched regarding the hydrangea's colors would be at all applicable to cannabis, this does support the supposition that the mechanisms at work could becommon to all anthocyanides.
In reading around, I saw that it is often recommended to raise the soil acidity by mulching the hydrangeas with hi-N stuff (grass clippings, coffee grounds,...). Yes, it's not the pH in itself, but we have all the minerals we'll ever need in the soil, all we need are the right microbes to mine them out.
So if initiating composting directly next to the plant will make hydrangeas go red, and @Rasta Roy 's microherd is diverse and healthy, we may see those pink trich's in his big-ass pot garden soon eh :bigjoint:
that shit is going to be dope!
 

Rasta Roy

Well-Known Member
Ohwow!
While at first I was very unsure whether what has been researched regarding the hydrangea's colors would be at all applicable to cannabis, this does support the supposition that the mechanisms at work could becommon to all anthocyanides.
In reading around, I saw that it is often recommended to raise the soil acidity by mulching the hydrangeas with hi-N stuff (grass clippings, coffee grounds,...). Yes, it's not the pH in itself, but we have all the minerals we'll ever need in the soil, all we need are the right microbes to mine them out.
So if initiating composting directly next to the plant will make hydrangeas go red, and @Rasta Roy 's microherd is diverse and healthy, we may see those pink trich's in his big-ass pot garden soon eh :bigjoint:
Fingers crossed! I had a couple ventilation set ups fail while I was up at the hospital with my wife (Crohn's disease). My room got down to 44 and 43 degrees on two different nights according to my monitor that tracks my temps. Gave em wicked cold stress. They'll live tho. About to finish rearranging and wiring my room so I can get my heat controls set up proper again. I've been running a half ass set up since I tore down my room after my last harvest. The lower temps have been delaying my root growth. I'm about to jump up to the big beds though, the roots are full enough and I'm getting impatient.
 

calliandra

Well-Known Member
Fingers crossed! I had a couple ventilation set ups fail while I was up at the hospital with my wife (Crohn's disease). My room got down to 44 and 43 degrees on two different nights according to my monitor that tracks my temps. Gave em wicked cold stress. They'll live tho. About to finish rearranging and wiring my room so I can get my heat controls set up proper again. I've been running a half ass set up since I tore down my room after my last harvest. The lower temps have been delaying my root growth. I'm about to jump up to the big beds though, the roots are full enough and I'm getting impatient.
Oooo but it sounds like you're pretty much ready to start them up! :D
I can imagine you're impatient, I'm all excited myself and it's not even my grow lol
Lots of good healing vibes to your wife too!
 

Rasta Roy

Well-Known Member
Oooo but it sounds like you're pretty much ready to start them up! :D
I can imagine you're impatient, I'm all excited myself and it's not even my grow lol
Lots of good healing vibes to your wife too!
Thank you! She was in there for five days and its been hell trying to spend time at the hospital, time at home with my dogs, and staying on top of work. Thank God it's the slow season.
 
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