Tissue Culture

Sour Wreck said:

based on the videos i have watched, a node was needed for each new growth area. in other words, with just one growing tip, can it be done?

Click to expand...

This is incorrect. Cannabis is totipotent which means you can regrow a whole plant from a single cell, yes, any cell. Most people use node scrapings.

There is a high end nursery here in Northern California called Dark Heart. They sell premium clones, standard plantlets and what they refer to as "Lablets" which are their in vitro TC clones.

Mycologists have been doing TC for ages. It is what a spore syringe is, liquid tissue media.

Don't go trying to re-invent the wheel,just tweak it if you need to. Here is the recipe I was going to try:

https://mycotopia.net/topic/50104-tissue-culture-protocol-for-mj/

Cleaning plants up to the mitochondrial level is generally not needed, nor possible to do at home.
If I was a giant corporation with some in-house developed strains, I would have that done professionally in order to have clean genetics to keep with the various banks.

can someone rec me a TC kit that has all the required chems? i know where to get sterile test tubes and clean room tools i just want a kit with HQ agar and all the hormones i will need

The single source that regularly shows up is Bill Graham out in the SFO/Oakland area. His website is microclone with the usual com at the end