Anyone used plant tissue culture to clone?

nickfury510

nickfury510

Well-Known Member
i know a guy that does this..he says it works for him...but i think il stick to the old fashioned way......
 
overfiend

overfiend

HeavyMetalHippie
i dont know about this one. there was a thread that people were trying to clone using leafs from pot plants and it diddnt work i think to successfully clone weed you need a stem with at least 1 node for new growth or it would be pointless.
big deal if you can keep a leaf alive for a month if it will never produce more than a leaf
 
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born2killspam

Well-Known Member
This is going to require extreme sterilization and environmental control in the early phase, but is very doable..
All plant cells are actually capable of undifferentiating, especially if auxin is abundant apparently, but the best callus formation would still come from undifferentiated root, or meristematic tissue..
Just like typical cloning, you'd be VASTLY more successful sarting with tissue we typically consider clonable, but with proper setup success with more differentiated tissue is far more probable with the sterility of this method..
Technically, this method could reproduce the plant that mothered a bag of chronic, but it would be far from the easiest tissue..
I might play around with this for giggles.. I have experience propagating yeast from trace sources in the home lab.. I'm not too keen on buying any kits though, I wonder how obtainable, or substitutable the culture constituents are..
 
overfiend

overfiend

HeavyMetalHippie
born2killspam said:
This is going to require extreme sterilization and environmental control in the early phase, but is very doable..
All plant cells are actually capable of undifferentiating, especially if auxin is abundant apparently, but the best callus formation would still come from undifferentiated root, or meristematic tissue..
Just like typical cloning, you'd be VASTLY more successful sarting with tissue we typically consider clonable, but with proper setup success with more differentiated tissue is far more probable with the sterility of this method..
Technically, this method could reproduce the plant that mothered a bag of chronic, but it would be far from the easiest tissue..
I might play around with this for giggles.. I have experience propagating yeast from trace sources in the home lab.. I'm not too keen on buying any kits though, I wonder how obtainable, or substitutable the culture constituents are..
Click to expand...
if you do play with this start a thread we'd like to see this kind of thing
 
M

muiks01

Active Member
Can be be done, did it once,still have everything to do it, but unless youre growing on a big scale,its really just a waste of time, way too much waste.
 
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born2killspam

Well-Known Member
List off what you got if you don't mind.. What type of tissue did you cultivate, and what would you describe as the difficulty level regarding sterilization/innoculation etc?
 
Jriggs

Jriggs

Well-Known Member
Do you think this meathod could be used on dry weed or will it only work on fresh leaves?

this is jsut hypothetical, was reading up on tissue cultore and came arcoss this thread -- sorry its a old bump.

but -- if you knew how to coudl i say go to another country that has a coffee house bring back a miniscule amount (say cheese) and if i had the correct enviroment grow it from that?
 
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born2killspam

Well-Known Member
Ever watch Jurassic Park?? Same concept applies.. If you can find a viable cell/dna then its theoretically possible, but that get harder to do as time goes by..
 
Grubs

Grubs

Well-Known Member
It works, it's doable, but not many people need that many plants. Normal cloning is too easy to go to the hassle.
 
Jriggs

Jriggs

Well-Known Member
i ws thinking more along the lines its an easy way to get some clone only strains from one country to another with little ot no risk. esp if oyu can take soem leaf from soem smoke and bring it back home to the us.
 
Grubs

Grubs

Well-Known Member
Ok, for that it makes sense. Snag a little uber-cool-nifty leaf and get clones.

I think it would have to be pretty fresh off the plant to do you much good.
 
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