Tissue Culture Cloning: eliminate mother plants & resources they require

Yooo, my fellow green thumbs,

DonTesla here from The Dons camp!!

Just creating a puddle where Micropropigation knowledge can pool..

a different style of cloning more suitable for small places and micro urban farms.. Plant tissue culture cloning or tissue culture or TC for short

Pro's:
-containers keep the plants small (size of container)
-disease, pest free conditions = healthy, sterile micro-clones every 4-6 wks
-Eliminates space and resources needed to keep mothers alive
-ideal not just for cloning, but storing, transporting and sharing
-TC has potential to induce polyploidy, a naturally occurring mutation that can unlock hidden genetics, (think mushroom trip or DNA activation vs GMO shit) inculding bigger and vigorous versions (super Mario!)

Any resources, links and know-how posts more than welcome ..

Btw, got a pressure cooker for sterilizing the culture we make
-understand the need to use forceps and scalpel in a sterile storage tote
-but don't know how to do it organically yet (agave recipe?) or exactly how they make the "sugar/jello/culture"
-And whether the rooting hormones are organic!?

But we gone figure it all out right here, right now..
That's the beauty of RIU

Trillions of trichs,
One love.

im very interested. ill be getting into this real soon....

greenghost420 said:

im very interested. ill be getting into this real soon....

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Good to know I'm not the only one..

If tissue could be preserved longer then I would see thos as an excellent way to build a strain library. Until then it seems like a lot of extra work when you ink of how fast cannabis grows and how easy it clones

I'm trying to find out how long an agar jar can go for, it sounds like its sustainable for decades .. Via a Multiplication vortex at least.

I think it depends on how quickly the culture is metabolizing the nutrients in the agar.

I imagine if kept in the cold they'd last a lot longer, not to mention obtaining a new agar dish and moving the culture to a new dish shouldn't be too much of an issue...

I'm curious to see if this is worth it too, considering the will of cannabis to survive and the ease of cloning.

I'll try to ask a lab tech about the agar.

This is such a bad ass concept that I want to start practicing. A guy told me about it a few years back, said that by doing this to a clone only strain it was like the hybrid vigor was brought back to level as a brand new seed being popped. I don't know how real genetic drift is but this guy was sure that TC eliminated it.

Great replies.. Lets myth bust this subject and find out the deets, nuances, and best way to ensure highest success rate and max length

@DonPetro yo dawg you are EVERYWHERE I look on RIU lol good to have you here too

a senile fungus said:

I think it depends on how quickly the culture is metabolizing the nutrients in the agar.

I imagine if kept in the cold they'd last a lot longer, not to mention obtaining a new agar dish and moving the culture to a new dish shouldn't be too much of an issue...

I'm curious to see if this is worth it too, considering the will of cannabis to survive and the ease of cloning.

Click to expand...

Cold temps is a big key to slowing things down, you got it, Fungus.. I think fridges are usually 4-6*C so that slows the metabolic processes down quite a bit, like how a mushroom trip can slow time right down..

If you got a lab tech in your circle that'd be real pimp..

Sparehead said:

This is such a bad ass concept that I want to start practicing. A guy told me about it a few years back, said that by doing this to a clone only strain it was like the hybrid vigor was brought back to level as a brand new seed being popped. I don't know how real genetic drift is but this guy was sure that TC eliminated it.

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Im calling BS.

Not on the fact that tc eliminates drift, but that cloning creates it.

A copy of a copy isnt exact, but when your splitting hairs down to a molecular level (and working with robust genetics), your full of shit if you say you notice a difference.


I have wondered if cloning off an unhealthy mother in sub optimal conditions over a lengthy period would somehow translate into a drift over time

Great thread, im kinda baffled by the concept. Lol just like with funghi petri cultures? Cut a piece onto substrate and watch it turn into a tiny plant?

hydroMD said:

Im calling BS.

Not on the fact that tc eliminates drift, but that cloning creates it.

A copy of a copy isnt exact, but when your splitting hairs down to a molecular level (and working with robust genetics), your full of shit if you say you notice a difference.


I have wondered if cloning off an unhealthy mother in sub optimal conditions over a lengthy period would somehow translate into a drift over time[/QUOTE

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I always try to take clones of healthy plants and I have not had any strains over 3 years (although have a nasty timewreck going 2 and some change). I can see how consistently taking clones from sick plants could lessen a cutting

I think the most important part of what the guy was talking about was restoring hybrid vigor through tissue culture...I probably just mixed in genetic drift myself I was pretty baked

I guess im confused how growing a cutting from a small piece of plant could do any better at restoring plant vigor than cloning... essentially its the same thing, except your using hormones to enduce both vegetative growth and roots... its
still cloning though.


Only benefit is being able to get hundreds of starts from a tiny mother if im not mistaken.

I have only successfully tissue cloned with material that is less than 2 months old. Even with refrigeration things seem to slow down over time.

Not to mention the 6 weeks it takes to get a seedling going makes for a long turnover

KLITE said:

Great thread, im kinda baffled by the concept. Lol just like with funghi petri cultures? Cut a piece onto substrate and watch it turn into a tiny plant?

Click to expand...

Essentially, but it takes a bit more nursing than inoculation a petree dish with fungus and letting it go!

You need a solution to facilitate the plant matter while adding correct hormones at proper doses to promote the growth you want.

I have a case study stashed somewhere on the subject from college. ill see about digging it out and posting

Essentially, but it takes a bit more nursing than inoculation a petree dish with fungus and letting it go!

You need a solution to facilitate the plant matter while adding correct hormones at proper doses to promote the growth you want.

I have a case study stashed somewhere on the subject from college. ill see about digging it out and posting

Click to expand...

Man i dreamt last night i had a fridge fuuuuuuuuuuuuuuuuull of petri dishes with crazy strain names. Pretty sure one had the word elf in it.
I imagined it would be a lot! harder than inoculating a funghi lol!
So if im getting it right you are meant to take ''stem cell'' tissue from the plant and find out what hormones or combination of hormones promote more stem cell growth in a medium that provides what it needs for a long time? I suppose the cold would make it inanimate once growth would be established?
Man who out there is doing actual research on this? I wouldnt mind helping with the funding tbh, especially after the dream i had man... Fridge full of dank...

pictures please or it didnt happend hard evidence is better to see and tell the diffrence can we see some pics

KLITE said:

Man i dreamt last night i had a fridge fuuuuuuuuuuuuuuuuull of petri dishes with crazy strain names. Pretty sure one had the word elf in it.
I imagined it would be a lot! harder than inoculating a funghi lol!
So if im getting it right you are meant to take ''stem cell'' tissue from the plant and find out what hormones or combination of hormones promote more stem cell growth in a medium that provides what it needs for a long time? I suppose the cold would make it inanimate once growth would be established?
Man who out there is doing actual research on this? I wouldnt mind helping with the funding tbh, especially after the dream i had man... Fridge full of dank...

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HAHAAAA! Elf!!! Man we need to see that come to fruition..
Elf's Haze..
Elf's Furry Dank
Elfindica

@KLITE
Elfphant Stomper (OG stomper cross)
Shit son, that's retro..

All day I'd hit them Elf-brids..

KLITE said:

Great thread, im kinda baffled by the concept. Lol just like with funghi petri cultures? Cut a piece onto substrate and watch it turn into a tiny plant?

Click to expand...

here's a pic to help us grasp these new thangs a bit..



Not sure how I missed this.. Lol

thump easy said:

... can we see some pics

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Presto..