Cannabis Genetic Modification: The basics .

Great thread SDS, wondering when you were going to get around again....miss the reading a little bit, just a teensie bit.

I am of two opinion with gmo's.

1, is that the technology and science behind GMO's, is very intriguing. Is it merely another tool, like when Linus Pauling was able to start isolating the molecular structure of chemical substances? Mutagenic mutation has been around for a while [Colchine treatments, silver sprays, etc etc] The strict structure of delineating genes [and there are a bunch] of every single strand of dna contained in each chromosome is fascinating imho....


2 What is done with GMO's, seems to teeter on the non-humanity scale. Food mainly being created as a by-product of genetic engineering seems to be created in the image of profit and not quality. Gmo food has never been touted as feeding the world, which I find ironic.

Correct me if am wrong though, that the dirty work is really the how and why genes are identified and then modified. It seems that gene sequencing is the main driver in this effort and it is coming along slowly, even for what has been identified. Knowing where and how the gene is located and turned on is due to the efforts of sequencing, then cutting a gene out with a lymase, is the byproduct of that knowledge.

....
And on top of that, I didn't see polyploidy discussed in the parts of the paper I did read and to sequence a triploid or tetraploid individual will be that much more work.

I honestly feel that part of the solution of eventual degradation of diploid Cannabis [if it ever occurs] is to branch into polyploidy species. RC Clarke mentions scientists that were modifying diploid individuals into polyploidy thru Colchine treatments, were getting higher yields of the monoterpenes in these varities.

Clarke also declares that polyploidy has not been found to be natural, but his work might be outdated in that area?

Very interesting stuff, especially when you come across this:

Here she/he is 2 weeks later, still throwing triple nodes after I cut a few of the leaves away and replanted this evening. Just trying to throw a little stress, to see how it reacts. I have heard this can be a phenotypic effect after a few nodes, so I am making sure, but hasn't thrown it yet.

I haven't pulled a male yet in this batch of seeds, good Fems.

Trillapods never yielded more for me as I'd heard.

Abiqua there is a lot of info on genomes and gene maps. It's the regulation and the interactions and roles of translated proteins that are still be discovered. Well tons to be discovered. But you could say it's like we have a map of an area and general locations for things but still not every detail is included on the map. It's interesting learning more about how exons and introns along with density regions with differing euchromatin to heterochromatin and methylation and other cool little things like micro RNA and siRNA. The technology behind sequencing is definitely not being. From the old days of manual Sanger sequencing to introduction of using labeled Dideoxy nucleotides with capillary electrophoresis with more fine matrices like with the emergence of polyacrlymide gels and now with the newer next gen sequencing you can do a lot. Our cancer panels we are designing in house for clinical use are pretty robust. 50 gene panels on an ion torrent. We have ran and done full exomes and that took about 3 days to have data. Which is way faster. I used to have to build and analyze cDNA libraries initially using a single capillary instrument that could only do s single injection and a couple hundred base pair reads at a time. When I got a 16 capillary I was so happy. Lol now we have a 96 capillary for our older sequencing stuff. We are supposed to get another semi conductor sequencer called the ion proton that can do 1-3 full human exomes per run up to 8 transcriptomes and even capable of full genome in about a week. I'll see if I can get the phone to upload some pics through tor in a minute.


That took forever to upload. Have pics of my 3130 and 3730 CE instruments too but man might hold off. Lol

SCARHOLE said:

Trillapods never yielded more for me as I'd heard.

Click to expand...

Triploid. Ploidy is the chromosomal number. But yeah I don't recall hearing really any benefit other than a cool little plant . Could be wrong though.

The three-leaf plants are often called triploid but it is a misnomer they are actually whorled phyllotaxy. They do tend to have closer internodes than their sisters but as you said, nothing unusual in terms of yield.

There's so much to be done and really, very little that we think we understand. Interaction networks are incredibly complicated and the basic principles behind many processes remain unknown. The research is also very time consuming...people can spend years on one protein alone.

@SupraSPL

I did jump the gun a bit inferring that a tricotyl maybe a result of polyploidy. Truthfully, I don't know. After reading thru the Int Industrial Hemp Journal archives and RC Clarke's Marijuana Botany, I thought I may have come across something printed, but I don't know for sure.

Anecdotally, I have heard that phyllotaxis maybe representative of a phenotype as well as a genotypic. Bruised Nuts, comes to mind, the only strain I have seen so far to pass the trait.

If it is phyllotaxis, that would interesting as well, because again anecdotally I have come across that most end up as males.

And I agree with your stance on GMO, I had to rethink my fascination comment. I am more in line with what @gkskunky wrote about simply understanding how the genes work in the genome. I am aware of the Purple Skunk /Chemdawg genomes that have been "completed".

GMO's are sponsored by corporations and entities that have left nothing but a wake of destruction from their use. Chemical warfare in Turkey and Armenia and then into WWI, IG Farben, Bayer as prominent examples in WWII, look at Monsanto's participation in Project Shadd during the Vietnam War and industrial chemical companies [who also have vertically integrated into agriculture] during the Iran/Iraq War and Kurdistan. Maybe not coincidentally many of these events also took place in mainly productive farmland areas as well.

Are seedless seeds [aka tissue culture's] considered a GMO product?

gk skunky said:

Abiqua there is a lot of info on genomes and gene maps. It's the regulation and the interactions and roles of translated proteins that are still be discovered. Well tons to be discovered. But you could say it's like we have a map of an area and general locations for things but still not every detail is included on the map. It's interesting learning more about how exons and introns along with density regions with differing euchromatin to heterochromatin and methylation and other cool little things like micro RNA and siRNA. The technology behind sequencing is definitely not being. From the old days of manual Sanger sequencing to introduction of using labeled Dideoxy nucleotides with capillary electrophoresis with more fine matrices like with the emergence of polyacrlymide gels and now with the newer next gen sequencing you can do a lot. Our cancer panels we are designing in house for clinical use are pretty robust. 50 gene panels on an ion torrent. We have ran and done full exomes and that took about 3 days to have data. Which is way faster. I used to have to build and analyze cDNA libraries initially using a single capillary instrument that could only do s single injection and a couple hundred base pair reads at a time. When I got a 16 capillary I was so happy. Lol now we have a 96 capillary for our older sequencing stuff. We are supposed to get another semi conductor sequencer called the ion proton that can do 1-3 full human exomes per run up to 8 transcriptomes and even capable of full genome in about a week. I'll see if I can get the phone to upload some pics through tor in a minute.


That took forever to upload. Have pics of my 3130 and 3730 CE instruments too but man might hold off. Lol

Click to expand...

Epic post, thanks.

This being the lighting forum and all, I wonder if @stardustsailor has come across any info on the genetic manipulation of photosynthesis, specifically in Cannabis?

It seems that most research is being conducted into manipulating the precursor's for increased or enhanced hydrocarboxyls.

University of Florida patent application

....IMPROVED AGRONOMIC TRAITS VIA GENETICALLY INDUCED ELEVATION OF PHYTOHORMONE LEVELS IN PLANTS

1. A method of producing a transgenic plant with Bgl overexpression relative to a wild-type plant, said method comprising: (a) introducing into a plant cell an expression cassette that comprises a Bgl gene to thereby produce a transformed plant cell; and (b) producing a transgenic plant from the transformed plant cell, wherein the transgenic plant has increased biomass, increased height, increased trichome density or increased seed production relative to a wild type plant….

9. A transgenic plant that overexpresses Bgl1 relative to a corresponding wild-type plant, wherein said transgenic plant has increased biomass, increased height, increased trichome density or increased seed production relative to a wild type plant….

15. The transgenic plant of claim 9, wherein said transgenic plant is Cannabis sativa, Papaver somniferum or Erythorxylum coca….

Ahhh, there it is. Saw this the other day while not logged in, who knew it'd be in the "LED and other lighting" subforum?

Just wanted to say: OMFG THIS IS TERRIFYING. But also intriguing. Probably more the former though.

Please don't let Monsanto ruin cannabis (or anyone else for that matter).

The Jurassic Park reference was perfect.

I have come across the whorled phenotype before, supposedly it is common in the UBC Chemo. I did come across an interesting mutation the other day I have never seen before. Its a leaf from my buddies outdoor grow, he took a cutting to sex it and I noticed the leaf. Its a male Asshole plant from Wild Rose Seeds he was gifted, notice the 2 smaller blades on top of the center one.