CannaWizard's (AMC) Lounge

W'sup Wiz? Long time, how's things?

cannawizard said:

*sup bro, yessum is right about the not concentrating on the branches snapping (like how all the books describe under "curing") since that usually leads to over drying your buds and you wont get the proper cure in the jars.. curing is still one of the hardest IMO to get right; takes years of practice and mistakes (trust me, i made many and i still dont have the science of curing down, hehe).. you got the right temps in the room; tho i'd drop the humidity to about 45-40% just to be on the safe side~ you want to get the outside of your budz crispy but the inside just a bit moist; so when you pop those into mason jars; you let the gasses build in the jars for about 24hrs; then you burp them (just pop the top and seal again).. next burp you should go for is 48hrs then another in 72hrs; it gets tricky unless you have those portable humidity gauges, since you want to stop burping the jars with about 30% hum in them... this is my method; there are TONS of curing methods out there.. goodluck buddy

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Interesting on how you let jars get to about 30% humidity. What some people have to say about curing is that it stops below 55%. what do you think?

Da Almighty Jew said:

Interesting on how you let jars get to about 30% humidity. What some people have to say about curing is that it stops below 55%. what do you think?

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*sorry bro, that was a typo on my end, sorry for that. I meant to say was 50% before the final burp of the jars (tho 55% would work), you dont want it too dry since that would stop the curing process ; glad you caught that~ usually i only do two burps before the long seal, i had that portable humidity checker to make sure lvls were okay before storing the jars.. miss cured buds, havent smoked anything properly cured for some time

sorry to hear that brother, im smoking some querkle, indica viper( john sinclair seeds), and og kush that has been cured for about a month. One hit gets me ripped.......Wish i could help you out but I'll take a nice bong rip for ya though.

tell me...can White Widow benifit anymore than usual with a UVB light?

Yes it can.. and I wish I could offer more than just a "yes & no" answer.. From what i've experienced growing WW, it does benefit from UVB exposure, exactly what happens when WW is exposed to extra UVB is still a mystery.. Unless someone steps up with a real lab with real PHds --doing tests-- then everything will remain "conjecture"

..cheers

**haven't been on RIU for awhile ., gonna clean up this thread a bit, finally found some time off from my 9-5.. actually working on a project to start growing coffee & tobacco (all depends if I can find the land to do it on) lol.. and maybe start a fertilizer biz.. just ideas for now.. hope all is well with everybody else.. peace

cannawizard said:

strain list:

-heres a bunch of strains that tested well with UV-b

..nirvana / K2
..serious / AK47
..thseed / mk-ultra
..dutch pass / power plant
..dr chronic / MP5k
..Ken's / GDP
..trichrome tech / purple kush
..southern california / og' (most of the kush strains respond beautifully to uvb, pure/landrace sativas on the other hand need snickers... lol

***any mj plant will benefit from UV-b, im just stating the ones that like it rough in bed, so-to-speak

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--update:

*serious seeds / motavation
*serious seeds / white rhino
*thseeds / cold creek kush
*htc / whitefire
*htc / headband
*sr71 / c99

..cheers

What up wiz I have been running a 500w blackstar flower led with a 600w hps in soil and feeding with organic Guanos and amendments. Ill try and post some pics if u like shit is getting frosty.

irieie said:

What up wiz I have been running a 500w blackstar flower led with a 600w hps in soil and feeding with organic Guanos and amendments. Ill try and post some pics if u like shit is getting frosty.

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**nice setup, yea feel free to post some frosty bud porn what strain you working on?

View attachment 2355093View attachment 2355094View attachment 2355095View attachment 2355096View attachment 2355097View attachment 2355098
(pics off ICmag / TheSG)

**collecting seeds of this sativa strain, it goes by many names but I like to call it "Kalinga".. I think it has great potential for its CBD+ , just assumptions until I get a batch going and tested~ I get migraines after an epileptic episode, but this strain just removes the pain after a couple of doobies.. it has potential.. just wanted to share some random tidbit~

..cheers

Ultra Violet update: UVR8 gene

UVR8 is an UV-B-sensing protein found in plants and possibly other sources.[SUP][2][/SUP] It is responsible for sensing ultraviolet light in the range 280-315 nm and initiating the plant stress response. UVR8 was first identified as a crucial mediator of a plant's repsonse to UV-B inArabidopsis thaliana containing a mutant this protein. This plant was found to have a hypersensitivity to UV-B [SUP][3][/SUP] which damages DNA.
UVR8 shares sequence homology with mammalian proteins involved in regulating chromatin condensation, for example the human RCC1 gene product, and has been shown to localise to the nucleus on exposure of the plant to UV-B light whereupon it interacts with chromatin.[SUP][4][/SUP] The protein has a beta-propeller fold[SUP][1][/SUP][SUP][2][/SUP] and is dimeric in the absence of UV-B light. It contains several tryptophan residues at the dimer interface which absorb light at UV-B wavelengths. This absorbance disrupts the interactions at the dimer interface, causing the dimer to dissociate and allowing interaction of the monomeric UVR8 with a protein called Cop1 E3 ubiquitin ligase.[SUP][5][/SUP] In turn, this triggers transcription of proteins responsible for UV protection and repair of UV induced damage


--sourced from Wiki

(Ultraviolet-B radiation (UV-B) is a key environmental signal that is specifically perceived by plants to promote UV acclimation and survival in sunlight. Whereas the plant photoreceptors for visible light are rather well characterised, the UV-B photoreceptor UVR8 was only recently described at the molecular level. Here, we review the current understanding of the UVR8 photoreceptor-mediated pathway in the context of UV-B perception mechanism, early signalling components and physiological responses. We further outline the commonalities in UV-B and visible light signalling as well as highlight differences between these pathways.)

--sourced from: Trends in Plant Science

cannawizard said:

Ultra Violet update: UVR8 gene

UVR8 is an UV-B-sensing protein found in plants and possibly other sources.[SUP][2][/SUP] It is responsible for sensing ultraviolet light in the range 280-315 nm and initiating the plant stress response. UVR8 was first identified as a crucial mediator of a plant's repsonse to UV-B inArabidopsis thaliana containing a mutant this protein. This plant was found to have a hypersensitivity to UV-B [SUP][3][/SUP] which damages DNA.
UVR8 shares sequence homology with mammalian proteins involved in regulating chromatin condensation, for example the human RCC1 gene product, and has been shown to localise to the nucleus on exposure of the plant to UV-B light whereupon it interacts with chromatin.[SUP][4][/SUP] The protein has a beta-propeller fold[SUP][1][/SUP][SUP][2][/SUP] and is dimeric in the absence of UV-B light. It contains several tryptophan residues at the dimer interface which absorb light at UV-B wavelengths. This absorbance disrupts the interactions at the dimer interface, causing the dimer to dissociate and allowing interaction of the monomeric UVR8 with a protein called Cop1 E3 ubiquitin ligase.[SUP][5][/SUP] In turn, this triggers transcription of proteins responsible for UV protection and repair of UV induced damage


--sourced from Wiki

(Ultraviolet-B radiation (UV-B) is a key environmental signal that is specifically perceived by plants to promote UV acclimation and survival in sunlight. Whereas the plant photoreceptors for visible light are rather well characterised, the UV-B photoreceptor UVR8 was only recently described at the molecular level. Here, we review the current understanding of the UVR8 photoreceptor-mediated pathway in the context of UV-B perception mechanism, early signalling components and physiological responses. We further outline the commonalities in UV-B and visible light signalling as well as highlight differences between these pathways.)

--sourced from: Trends in Plant Science

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So there is a protein that can detect when the plant is exposed to UV light that's really amazing...So here some food for thought so plants grown out side need this protein in order to help better adapted survive in natural light conditions ..right so lets say a strain was to be grown and breed over and over again indoor under grow lamps and a low UVB environment ..Do you think to is possible that the plants could lose or turn off this gene expression of this protein because it was no longer used or need indoors ..So lets say 80 years of being seeded and bred indoors if you brought the plants outside your grandson wanted to grow outdoors with some old seeds he got in your will ..could it be possible that the plants would be unable to grow in sunlight ..that the had changed and adapted to grow to only grow in an artificial environment under low uvb artificial lighting??????

o and cannawizard since your darth vader im going to be yoda for a while

billy4479 said:

So there is a protein that can detect when the plant is exposed to UV light that's really amazing...So here some food for thought so plants grown out side need this protein in order to help better adapted survive in natural light conditions ..right so lets say a strain was to be grown and breed over and over again indoor under grow lamps and a low UVB environment ..Do you think to is possible that the plants could lose or turn off this gene expression of this protein because it was no longer used or need indoors ..So lets say 80 years of being seeded and bred indoors if you brought the plants outside your grandson wanted to grow outdoors with some old seeds he got in your will ..could it be possible that the plants would be unable to grow in sunlight ..that the had changed and adapted to grow to only grow in an artificial environment under low uvb artificial lighting??????

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--what you stated is plausible.. I'm not sure if a gene could be turned off by selective breeding indoors, and it could probably be a factor later on since UVb levels are increasing outdoors.. But seeing how resilient this plant is, I doubt it will ever loose its affinity being grown under natural sunlight.. Like I said, what you presented is plausible

--haha nice avatar

UVR8 (cont)--

Ultra-violet radiation, particularly UVb, has been shown to be detrimental to plant cell function. UV causes damage to plant cells in the same way it tears through your skin, eventually causing sunburn. As a result of this damage, all plants have created defenses against UV in the form of gene UVR8.

UVR8 is a protein molecule which senses UV all by itself, and then "tells" plant cells to change their behavior. Exactly how UVR8 molecules sense UV was recently discovered and is pretty interesting. UVR8 is what chemists call a "dimer." which just means that it's made of two structurally similar protein subunits. When UV light hits the two protein subunits in UVR8, their charge weakens and they break apart. To help visualize this, imagine rubbing two balloons against one another. The balloons will stick together because of a static charge. Now imagine the balloons get rained on. The water takes the static charge with it and the two balloons fly apart. In this example, the balloons are the two protein subunits and the rain is UV light cascading down on the plant cell. After the protein subunits break apart, they head to the cell nucleus to deliver their information.

One of these changes caused by this information is very important in your cannabis garden. UV stress stimulates cannabis' production of chemicals via the phenylpropanoid pathway, specifically malonyl-CoA and phenylalanine. Why is this important? It's important because cannabis uses malonyl-CoA to make Olivtol, which it in turn uses to make THC. Now we can see the specific pathway which cannabis uses to increase potency when exposed to UV light.

**which still leads me to question, if they are suggesting having more THC sites / resin-terpes equate to higher potency??

(sourced from: http://bigbudsmag.com)

--The only thing I don't agree with concerning the write-up on "Using UV light on MJ" was the part about adding MH bulbs to boost UVb levels..

here's is a simple brew for your rhizosphere:
(not feeding the roots but the microbes around them)

7gal RO water
(.6oz) fish hydrolysate
(.8oz) soluble kelp
(.5oz) humic acid
(3-4cups) gh ancient forest (put in cheesecloth)
(1tbsp) sucanat
(1tbsp) brown rice syrup

--bubble for 24-36hrs, and feed
(don't forget to stir)

(you could add extra bacteria/fungi from all the products out there, but if your using soil, im assuming the micros are already present.. but if your hard pressed on adding extra bac/fun --it won't hurt)

I use amended FFoF as my medium~

#cheers #elaineingham

whats up Wiz. Long Time.

so how much UVB would be considered good supplemental per square ft/ hours per day?

**2 beers open, out of wax have a lil herb, bout to trim these 4 purple bitches!!!

grapeoptimo said:

so how much UVB would be considered good supplemental per square ft/ hours per day?

**2 beers open, out of wax have a lil herb, bout to trim these 4 purple bitches!!!

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*Sup bro, been a minute hasnt it , goonies never say die!! haha --damn haven't smoked any good purp strains for awhile (miss those indys)

--about the UVb levels, since nothing is really solid fact just yet concerning this topic, I would just stay around the 250µW/cm² - 450µW/cm² (microwatts per square centimetre) --which is pretty much mimicking outdoor levels Some strains with certain phenos can handle past 500µW/cm² --but thats just hearsay

hours? just have them on when your lights are on, (12/12/,10/14).