i assume you are running hid lighting and the fan is to cool the light and if this is the case you should run it the entire time the lights are on. if the fan is simply to exchange/filter the air it's really up to you. my timer has 15 min increments so i would start 45 min off 15 on and so on, so the air would be injected/exhausted once an hour, which "should" be sufficient for a closet that size. you would have to see what happens and make adjustments accordingly. if it was still too stuffy/hot then you could exchange the air more frequently.