More THC testing – UVA vs UVB vs near-UV

ANC

Well-Known Member
We took four samples of the same strain, all grown in coco run-to-waste by competent growers under slightly different lighting set-ups, with two of the samples being provided by the same grower under the same conditions, but grown six months apart (to compare the ageing process or "cure"). This experiment sought to duplicate, or at least compare with, an earlier test conducted by @Or_Gro and posted up by @Prawn Connery here: https://www.rollitup.org/t/thc-cbd-terpene-test-results-uva-vs-uvb-vs-none.1001617/

The strain was a local eight-week Afghan-Skunk variety that had been shared amongst three growers. This makes the experiment a little bit more variable than the Or_Gro test, but it was the best we could do locally given legal constraints. The samples were marked "A", "B", "C" and "D" and the tester was not provided with any other information.


Sample A
Grown under eight Grow Lights Australia "High Red" boards with 4x Arcadia 6/30% UVB/UVA lamps running 12 hours a day (full 12/12 cycle) averaging 1000 PPFD. Coco run-to-waste, Canna nutrients. Here is the spectrum.
View attachment 4521007


You cannot see the UVB in the above spectral reading, but here is what the 6% Arcadia bulb looks like.
View attachment 4521018



Sample B
Four High Light UV boards averaging 1000 PPFD. Coco run-to-waste, Canna nutrients. This sample was about seven months old. Here is the spectrum.
View attachment 4521008



Sample C (same as Sample B)
Four High Light UV boards averaging 1000 PPFD. Coco run-to-waste, Canna nutrients. This was a fresh sample less than one month old. Here is the spectrum.
View attachment 4521008



Sample D
Two High Red Boards, two 315CMH lamps, averaging 1000 PPFD. Coco/perlite run-to-waste, Hygen nutrients. Here is the spectrum.
View attachment 4521013



Samples B and C were grown and provided by @Prawn Connery, who also did the PPFD testing and supplied the spectrographs. Sample A was grown by a local RIU member, and Sample D was grown by a friend of Prawn's.
I'm afraid the 6 months lag is invalidating your results to some degree.
I grow the same cultivar year in and out, and it changes cyclically through the year as if the plants are still aware of what season it is. outside.
Best would be to run three tents and do your tests at the same time. This is also the only way that you can achieve guaranteed standardised feeding for all groups.
 

Grow Lights Australia

Well-Known Member
exactly and therefore it may seem a good idea to instead go for the blue bandwith as youll be delivering PAR instead of UV heat, but Im afraid your trading weight for quality, in fruits even greater health potential. The secondairy plant metabolites do also affect taste & scent.

I wonder why green 550nm isnt such an attractiv wavelength to outfit boards. Since its more penetrating, it will reach deeper into the plants and cause less top heat. Not that I know much about available chips on the market...

btw the graphic is broken
I hope it is fixed now. Green is interesting – we know we need it – but it appears that everything is a trade-off and we do know that too much green is detrimental to pant health (and can even kill some plants that would otherwise grow under red or blue or both combined), so it is probably a matter of finding the right balance. Or introducing under-canopy lighting to make up for the lack of green penetration if it proves that blue and especially red give us much more bang for buck (absorbed photons vs energy required).
 

Grow Lights Australia

Well-Known Member
I'm afraid the 6 months lag is invalidating your results to some degree.
I grow the same cultivar year in and out, and it changes cyclically through the year as if the plants are still aware of what season it is. outside.
Best would be to run three tents and do your tests at the same time. This is also the only way that you can achieve guaranteed standardised feeding for all groups.
I would agree (that is exactly what happened in the earlier Or_Grow tests), even though most of the recent tests we did were quite close to each other. The six-month sample was deliberately included to see how the same strain aged. The other three samples were grown within two months of each other.
 

Grow Lights Australia

Well-Known Member
Could it be that the plant gets more uvb in early to mid flower in nature but you added it thru all flower stages? The precursor to thc, cbg is generated with a higher cct. So too much uvb which put the plant into early to mid flower while your timeline for 12/12 was more mid to late flower. Meaning your timeline for flower didnt match the plants timeline for maturing and converting cbg to thc.
Again, that is possible. The Or_Gro experiment resulted in the non-UV tent finishing a week earlier with the same yields as the UV tents.
 

Bignutes

Well-Known Member
It's true. And this is what makes me wonder about 285nm, as there is almost none in natural sunlight and even then only the most minute part in high UV areas, such as the equator (admittedly where cannabis evolved). I'm sure you wouldn't need much of it to see an effect. But I'm not sure how much effect is to be had and the risks vs rewards are would need to be weighed. I'm not about to rule something out tat we haven't tried ourselves.

View attachment 4533542
I wouldn't want to build a light where the longevity of the 285 to 350ish nm burn out quick relative to the other wavelengths, your customers would be upset, but I understand your intention is good. Also leaving even a small amount of 265 all day burns up the plants which then leaves you with making separate leds with separate schedules, big PITA. To sum it up it's not practical from both the mfg to the end user. A good balance would be what i described above 400-430 and 740 in the right ratio, this gives you better lifespan and you can leave it on all day for one light, one schedule. The most bang for your buck is looking at what pigments absorb what wavelength and focusing on those. I don't see 265 to 350 being relevant, a little more between 350 and 400 but not as important as 400+. I would address the dip at 480 before anything below 350.

If you were to put the light wavelengths and apply them to where they are most applicable and readily utilized by the plant this will serve two purposes. The first is your light efficiency as it applies to the plants response could potentially set the bar for light standard in horticulture. The second is you could advertise a lower operating cost on you electricity bill. You could end up with a light that grows equally well at 24 watts per sq ft versus the 30 now. That's where the real market is at.

Providing the end user with the mostest for the leastest makes for a happy customer and manufacturer.
 
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hybridway2

Amare Shill
It's true. And this is what makes me wonder about 285nm, as there is almost none in natural sunlight and even then only the most minute part in high UV areas, such as the equator (admittedly where cannabis evolved). I'm sure you wouldn't need much of it to see an effect. But I'm not sure how much effect is to be had and the risks vs rewards are would need to be weighed. I'm not about to rule something out tat we haven't tried ourselves.

View attachment 4533542
Just a very small amount to trigger the synergistic effects so you can see them when comparing. After adding 365 + 385.
A nice slow ramp upwards to the 450, then a nice slope downward with 470 into green. You'll have all bases covered needed to see what happens in comparison to everything.
Why not start with the fullest spectrum as your test against tool & then go from there?
 

hybridway2

Amare Shill
I wouldn't want to build a light where the longevity of the 285 to 350ish nm burn out quick relative to the other wavelengths, your customers would be upset, but I understand your intention is good. Also leaving even a small amount of 265 all day burns up the plants which then leaves you with making separate leds with separate schedules, big PITA. To sum it up it's not practical from both the mfg to the end user. A good balance would be what i described above 400-430 and 740 in the right ratio, this gives you better lifespan and you can leave it on all day for one light, one schedule. The most bang for your buck is looking at what pigments absorb what wavelength and focusing on those. I don't see 265 to 350 being relevant, a little more between 350 and 400 but not as important as 400+. I would address the dip at 480 before anything below 350.

If you were to put the light wavelengths and apply them to where they are most applicable and readily utilized by the plant this will serve two purposes. The first is your light efficiency as it applies to the plants response could potentially set the bar for light standard in horticulture. The second is you could advertise a lower operating cost on you electricity bill. You could end up with a light that grows equally well at 24 watts per sq ft versus the 30 now. That's where the real market is at.

Providing the end user with the mostest for the leastest makes for a happy customer and manufacturer.

I used to be head of R&D for new product design this kind of stuff is my wheelhouse.
Its gotta be a split the difference type if deal. Due to tech. Restraints
 
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Grow Lights Australia

Well-Known Member
I wouldn't want to build a light where the longevity of the 285 to 350ish nm burn out quick relative to the other wavelengths, your customers would be upset, but I understand your intention is good. Also leaving even a small amount of 265 all day burns up the plants which then leaves you with making separate leds with separate schedules, big PITA. To sum it up it's not practical from both the mfg to the end user. A good balance would be what i described above 400-430 and 740 in the right ratio, this gives you better lifespan and you can leave it on all day for one light, one schedule. The most bang for your buck is looking at what pigments absorb what wavelength and focusing on those. I don't see 265 to 350 being relevant, a little more between 350 and 400 but not as important as 400+. I would address the dip at 480 before anything below 350.

If you were to put the light wavelengths and apply them to where they are most applicable and readily utilized by the plant this will serve two purposes. The first is your light efficiency as it applies to the plants response could potentially set the bar for light standard in horticulture. The second is you could advertise a lower operating cost on you electricity bill. You could end up with a light that grows equally well at 24 watts per sq ft versus the 30 now. That's where the real market is at.

Providing the end user with the mostest for the leastest makes for a happy customer and manufacturer.
As a company, I would not only have to agree, but also point out that what you're suggesting is what we have been trying to achieve all along by providing a balanced spectrum with few trade-offs in terms of yield, essential oil production, efficiency and longevity. There is also usability and cost, which is the reason for putting the entire spectrum on one channel (no extra drivers or supplemental spectra needed), as well as the focus on producing a dedicated flowering board as that is the aim of nearly all cannabis growers.

Regardless of the above, there are already manufacturers producing supplemental lighting products so there is no shortage of options for people to try. It is not our place to knock other manufacturers or their work. We're just trying to make the best alround flowering lights we can.
 

Grow Lights Australia

Well-Known Member
Just a very small amount to trigger the synergistic effects so you can see them when comparing. After adding 365 + 385.
A nice slow ramp upwards to the 450, then a nice slope downward with 470 into green. You'll have all bases covered needed to see what happens in comparison to everything.
Why not start with the fullest spectrum as your test against tool & then go from there?
Well yes, the idea is really to produce a spectrum with no holes in it and peaks in the right places where flowering plants can use them. It does appear though that there may be a point where you trade off dry yield for canabinoid yield by trying to balance the amount of red, blue and especially UV if you want to go that route. I know there are some companies like Apogee saying spectrum doesn't make a difference to cannabinoid content, but that is not what we have been seeing.
 

hybridway2

Amare Shill
Well yes, the idea is really to produce a spectrum with no holes in it and peaks in the right places where flowering plants can use them. It does appear though that there may be a point where you trade off dry yield for canabinoid yield by trying to balance the amount of red, blue and especially UV if you want to go that route. I know there are some companies like Apogee saying spectrum doesn't make a difference to cannabinoid content, but that is not what we have been seeing.
Yes, 100% Agree, its all about balance.
APOGEE only says that now becauae they have NO "Scientific Data" proving otherwise By a well known Scientific. Why? Because they haven't done the tests!..Same applies to his conclusion to ir. Any Horticulturalist will tell you different. But who are they right? Seeing how their citings & studies are constantly ignored by several led growlight companies, its only the scientists that matter to some.
We are several steps ahead of where he's testing now but he'll catch up quick & update his videos.
You are making waves my man!
Keep up the good work!
 
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Kassiopeija

Well-Known Member
Well yes, the idea is really to produce a spectrum with no holes in it and peaks in the right places where flowering plants can use them. It does appear though that there may be a point where you trade off dry yield for canabinoid yield by trying to balance the amount of red, blue and especially UV if you want to go that route. I know there are some companies like Apogee saying spectrum doesn't make a difference to cannabinoid content, but that is not what we have been seeing.
may I ask if the spectrum should be optimized for a specific plant like Cannabis or be generally to grow all plants?
 

Kassiopeija

Well-Known Member
Well yes, the idea is really to produce a spectrum with no holes in it and peaks in the right places where flowering plants can use them.
I wonder if just peaks would be better...? because some of the metabolites have multiple highly reactive tops. Maybe you can therefore narrow down the number of different diodes being used.
could maybe rely then on more efficient bandwiths...
 

FADING-SILHOUETTE

Well-Known Member
No, as @wietefras said CBG is the precursor to CBGa (the acidised form) which is the precursor to other cannabinoids such as THC and CBD.


There is a good amount of easy-to-read information here: https://darkgreensingularity.com/cannabis-production-science-research/

And here: http://biology.mcgill.ca/Phytotron/LightWkshp1994/3.3 Hashimoto/Hashimoto text.htm

The TLDR version is that 365nm is a sub-peak of <450nm spectral peaks including cryptochrome and phototropin, as well as phytochrome red (Pr), so it is believed to either have a synergistic effect when combined with those primary peaks, or may replace them in their absence (read below).

An example of this is in the second link above which details an experiment where spinach was grown with and without UV(A/B) light. Broad-spectrum UVA/B was found to inhibit growth, whilst the addition of 365nm (with no other UV) promoted growth. UV has been proven to counter shade-avoidence, which is controlled predominantly by the Red:Far Red ratio. This would make sense if the 365nm absorbance peak has a synergistic effect to convert higher amounts of Pr to increase the R:FR ratio in plants. But it has also been shown to inhibit growth and yields.

Looking at the graph below you can see cryptochrome peaks at 405nm, whilst there is a secondary phytochrome Far Red peak also at 405nm. So it seems UVA (365nm) works best in combination with blue light <450nm. Also, if the desire is to increase the Emerson Effect (synergistic relationship between Pr and Pfr), then perhaps the same can be achieved with the addition of 405nm, as LEDs usually have a very high R:FR ratio anyway. In which case you may not want the synergistic effect of 365nm that would otherwise increase the R:FR ratio. Sunlight has a ratio of about 1:0.7 R:Fr (it changes through the day and seasons), whilst typical 450nm peak "blue pump" LEDs have a ratio closer to 6 or 7:1 – which is very high when you think about it. And probably why plants grow a lot shorter and denser under LED than other forms of light.

As to THC and other cannabinoids, there are test results here showing a combination of 285nm (UVR8 ) and 365nm increases THC: https://www.agricultra.com/uvr8-chemical-profile-shaping-in-cannabis

However, the results appear marginal (2-11% increase) and the most interesting thing about those tests is it appears there was no near-UV or deep blue light in the overall spectrum <450nm – so would it perform better with the addition of near-UV, or would near-UV alone be sufficient? The Or_Grow test (and other scientific tests such as this one: https://www.karger.com/Article/Fulltext/489030) showed that the addition of <450nm light increased cannabinoids by at least 20%, whilst further testing is starting to show the same results: that near-UV can have as much effect (or more) on cannabinoid production as the addition of UVA and UVB and possibly without impacting yields. (I say "possibly" as we haven't really proven it, even though the near-UV tests also outyielded the UVA/B tests.)

There's a lot going on between 400nm and 450nm and very few LEDs cover this range. So if results with 365nm do show an improvement in growth, cannabinoid or terpene levels, then how do we know that 365nm is not simply making up for the lack of 400-450nm in typical leds in the first place? Perhaps the real test is to add 365nm as well as 400-450nm.

View attachment 4533544
This is my current setup.. I kinda guessed what I needed ... but so far it's all working really well ... I have a UVB T8 in there too and 2x 365nm UV 36w LED lamps ...along side of a 660nm far red and ir lamp etc... - STELTHY :leaf:20200403_154800.jpg20200408_153859.jpg20200412_151226.jpg

*Shes just started the 4th week since 12/12..
 
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Grow Lights Australia

Well-Known Member
may I ask if the spectrum should be optimized for a specific plant like Cannabis or be generally to grow all plants?
The obvious answer would be that if you are designing a flowering lamp specifically for cannabis, then it should be optimised for cannabis. Growers have already pointed out that a lot of horticultural scientific tests are done on leafy greens with a focus on increasing leaf mass instead of flowering mass. We know blue light makes leaves thicker – great if you are growing lettuce – and that increasing amounts of far red light can cause stretch as well as thinning and broadening of leaves, but at the same time increase overall yields (thinner leaves with more surface area can photosynthesise more). One of the problems is there hasn't been a lot of research specifically into cannabis so we are constantly learning.

For our part we built a light based on what growers had been observing in terms of yields and finishing times related to spectral differences between HIDs (HPS and CMH) and different types of LEDs. I believe one of @Prawn Connery's friends had seen faster finishing times while growing under HLG's now discontinued QB324 boards that had Nichia CRI90 LEDs and a fair bit more red and far red compared to typical CRI80 3000K LED boards. That's where Prawn got the idea for the High Light boards. Other research into the effects of near-UV led us to add this to the spectrum.

Ultimately the future of LEDs will be tuneable spectra for different stages of growth as well as different species of plant. It's true that all plants evolved under the same sun, but the sun's spectrum changes in different parts of the world so maybe we should be focussing on what the sun is doing at the equator or in Afghanistan if we want to unlock the secrets of cannabis. We do know that UV is higher at the equator and that light is not as "blue" because there is more ozone and higher humidity (more water in the atmosphere) that scatters blue light (it also scatters UV, but because there is a higher concentration of light at the equator due to the sun hitting the earth at a less oblique angle, the concentration of UV is higher). We know the sky is "bluer" in Afghanistan because it is higher and there is less ozone. When you look at a typical sativa plant it appears to be a lighter shade of green or "yellower" than an indica, which appears darker or "bluer". Is that because indicas evolved in high UV and high blue light compared to sativas which evolved in high UV and low blue light? Indica leaves appear to reflect more blue light than sativas. That is the apparent environmental difference in just one species of plant so the answer might not be that simple.
 

Prawn Connery

Well-Known Member
I'm afraid the 6 months lag is invalidating your results to some degree.
I grow the same cultivar year in and out, and it changes cyclically through the year as if the plants are still aware of what season it is. outside.
Best would be to run three tents and do your tests at the same time. This is also the only way that you can achieve guaranteed standardised feeding for all groups.
Hey mate, apart from the 7-month sample – which was mine – the UVB sample was no more than two months behind the others, which were two weeks apart.

I know what you mean about plants growing differently indoors at different times of year, but I always put that down to the change in temperature and humidity. If I had perfect climate control, I'd expect them to grow the same all the time. I mean, a seed doesn't know if it's germinating in the northern or southern hemisphere, right? So how would it know what the conditions are like outside if it wasn't at least influenced by them indoors? Pheromones from other plants? I don't know.
 

Prawn Connery

Well-Known Member
This is my current setup.. I kinda guessed what I needed ... but so far it's all working really well ... I have a UVB T8 in there too and 2x 365nm UV 36w LED lamps ...along side of a 660nm far red and ir lamp etc... - STELTHY :leaf:View attachment 4533959View attachment 4533961View attachment 4533962

*Shes just started the 4th week since 12/12..
Cool set-up mate. It's a bit ghetto but I like it :bigjoint:

What's the circular LED fixture in the middle with the white mid-powers and red, blue and far red monos under a heatsink?
 

Rocket Soul

Well-Known Member
No, as @wietefras said CBG is the precursor to CBGa (the acidised form) which is the precursor to other cannabinoids such as THC and CBD.


There is a good amount of easy-to-read information here: https://darkgreensingularity.com/cannabis-production-science-research/

And here: http://biology.mcgill.ca/Phytotron/LightWkshp1994/3.3 Hashimoto/Hashimoto text.htm

The TLDR version is that 365nm is a sub-peak of <450nm spectral peaks including cryptochrome and phototropin, as well as phytochrome red (Pr), so it is believed to either have a synergistic effect when combined with those primary peaks, or may replace them in their absence (read below).

An example of this is in the second link above which details an experiment where spinach was grown with and without UV(A/B) light. Broad-spectrum UVA/B was found to inhibit growth, whilst the addition of 365nm (with no other UV) promoted growth. UV has been proven to counter shade-avoidence, which is controlled predominantly by the Red:Far Red ratio. This would make sense if the 365nm absorbance peak has a synergistic effect to convert higher amounts of Pr to increase the R:FR ratio in plants. But it has also been shown to inhibit growth and yields.

Looking at the graph below you can see cryptochrome peaks at 405nm, whilst there is a secondary phytochrome Far Red peak also at 405nm. So it seems UVA (365nm) works best in combination with blue light <450nm. Also, if the desire is to increase the Emerson Effect (synergistic relationship between Pr and Pfr), then perhaps the same can be achieved with the addition of 405nm, as LEDs usually have a very high R:FR ratio anyway. In which case you may not want the synergistic effect of 365nm that would otherwise increase the R:FR ratio. Sunlight has a ratio of about 1:0.7 R:Fr (it changes through the day and seasons), whilst typical 450nm peak "blue pump" LEDs have a ratio closer to 6 or 7:1 – which is very high when you think about it. And probably why plants grow a lot shorter and denser under LED than other forms of light.

As to THC and other cannabinoids, there are test results here showing a combination of 285nm (UVR8 ) and 365nm increases THC: https://www.agricultra.com/uvr8-chemical-profile-shaping-in-cannabis

However, the results appear marginal (2-11% increase) and the most interesting thing about those tests is it appears there was no near-UV or deep blue light in the overall spectrum <450nm – so would it perform better with the addition of near-UV, or would near-UV alone be sufficient? The Or_Grow test (and other scientific tests such as this one: https://www.karger.com/Article/Fulltext/489030) showed that the addition of <450nm light increased cannabinoids by at least 20%, whilst further testing is starting to show the same results: that near-UV can have as much effect (or more) on cannabinoid production as the addition of UVA and UVB and possibly without impacting yields. (I say "possibly" as we haven't really proven it, even though the near-UV tests also outyielded the UVA/B tests.)

There's a lot going on between 400nm and 450nm and very few LEDs cover this range. So if results with 365nm do show an improvement in growth, cannabinoid or terpene levels, then how do we know that 365nm is not simply making up for the lack of 400-450nm in typical leds in the first place? Perhaps the real test is to add 365nm as well as 400-450nm.


That made a nice read. I think theres a point in mentioning re plant health that apart from finishing times, yields and cannabinoids uv (a,b and near) is also related to the opening of the stomata which seems vital when growing under leds. Theres an abundance of information (but not always cannabis specific) under the google search term "stomata aperture action spectrum"
 

Prawn Connery

Well-Known Member
Could it be that the plant gets more uvb in early to mid flower in nature but you added it thru all flower stages? The precursor to thc, cbg is generated with a higher cct. So too much uvb which put the plant into early to mid flower while your timeline for 12/12 was more mid to late flower. Meaning your timeline for flower didnt match the plants timeline for maturing and converting cbg to thc.
It's an interesting concept and you could be on to something. The only doubt in my mind would be that HIDs (especially metal halide) also throw out a small amount of UVB throughout the cycle which doesn't seem to affect flowering times. The other consideration is that when I spoke to the grower he said he looked at the triches under a loupe prior to harvest and they were mostly milky turning amber. I would expect clear trichomes to have higher levels of CBG, but not mature trichomes. However, I've never tested the theory.

This is the trouble with cannabis being illegal for so long: there are so many "wives tales" and so little scientific information, as GLA has already mentioned, that it's hard to know what's real and what's not.
 

Barristan Whitebeard

Well-Known Member
Cool set-up mate. It's a bit ghetto but I like it :bigjoint:

What's the circular LED fixture in the middle with the white mid-powers and red, blue and far red monos under a heatsink?
That is an Optic LED Phantom 1XL. It's a 104 watt light that uses a Mean Well HBG series driver. The monos are Cree XP-E deep red and royal blue.

It's a pricey light. $239 US dollars.
 
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