That magic moment

I've spent a couple of months going through old mycelium that kept contaming, then finally ordering needles, then going through a couple of stupid contam issues (my own, old old grain, too short PCing), then FINALLY 8 test trays got layed out and given a 2:1 (grain:sub, I wanted fast), and 6 of those contamed (way too much moisture), then I got that right, and then those fuckers showed primordia (huge path of it) for 3 f'ing weeks before the 1st pin just showed up.

YAYAYAYAYAY.

No pin porn, sorry.

Too long, primordia should show up only a few days before pins in most species, except shaggys, that takes two weeks to go from "dials" (the way they start up - little raised disks) to pins - protrusions from the middle of the "dials".


maybe your temps are low?

canndo said:

maybe your temps are low?

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68 night - 73 day.

I think it was more of a humidity problem. I ran low (not near empty though) in my bucket and did not realize the cables to the floating pond fogger were tight. I had another cool mist going so it never dropped under 80%, but that might have been enough. That was a week ago.

These trays are merely eye candy while I wait for the tubs to mature, I'm not worried about them.

Question to you on myc storage.

Let's say I PCed a small amount of corn in a jar that had 2 RTV seals (no air filter, on purpose).
When done PCing (2+ hours, 15-19 psi), this jar would have a near vacuum.
Then I would have a needle filled with GLC.
Placed into this container, the needle would empty (many times over actually).
But in this case, I just have a little in the needle so it gets a good noc dosage.

I would NOT expect this to grow. Near vacuum? How about you?

I would expect to be able to store this away (no refrigeration required) and introduce air via air port needle sometime in the future to kick start the growth.

Is this a fantasy or a reasonable storage method?

testtime said:

68 night - 73 day.

I think it was more of a humidity problem. I ran low (not near empty though) in my bucket and did not realize the cables to the floating pond fogger were tight. I had another cool mist going so it never dropped under 80%, but that might have been enough. That was a week ago.

These trays are merely eye candy while I wait for the tubs to mature, I'm not worried about them.

Question to you on myc storage.

Let's say I PCed a small amount of corn in a jar that had 2 RTV seals (no air filter, on purpose).
When done PCing (2+ hours, 15-19 psi), this jar would have a near vacuum.
Then I would have a needle filled with GLC.
Placed into this container, the needle would empty (many times over actually).
But in this case, I just have a little in the needle so it gets a good noc dosage.

I would NOT expect this to grow. Near vacuum? How about you?

I would expect to be able to store this away (no refrigeration required) and introduce air via air port needle sometime in the future to kick start the growth.

Is this a fantasy or a reasonable storage method?

Click to expand...

Your temps are low. It is wise to lower your temp to "set" the pins but they should be raised again after a few days to high 70's or low 80's. Ameteurs, you do NOT need to do this, temperature changes are simply for fully orchestrated flushes and your mushroom patch will do just fine without such adjustments.

you will still get growth at 80 percent but you might get split or cracked caps - remember that there is a microclimate close to your casing that will keep the pins moist.

I believe it will germinate even in a near vaccum. Initialy your myclium needs very little oxygen and produced copious amounts of co2 (a bag is enough to change the ppm in a 10 x 10 room).

Then, Well, I don't know test. I have been working on mycelium storage for 20 years and have yet to find a satisfactory method.

storage in sterile water in the fridge works only for a few months
Storage under sterile oil is a big pain in the ass
It is said that perfectly dried mycelium, when sprinkled on agar will "come back to life" - not for me
A quart jar, 3/4 filled with mycelium and sealed will die completely in three months
That same jar with a filter lid will die in about 6 if the temperatures are low
The only way I have found is active storage - where a bit of growing mycelium is taken from a dish and placed on another dish but the limitation there is absolute length of the mycelium. The longer the mycelium is the older it is and eventualy the genetics will be ruined even if the medium is changed - and this is a real pain. I have lost any number of unique strains, land races, subspeices, whatever - and I am fairly certain I came across a sclerotia forming cubensis even though it is thought not to be possible - but we will never know.

And Shaggy mycelium are even more volutile, damn it.

So, my grow bags are actually supplying my weed plants with CO2?!

canndo said:

Your temps are low. It is wise to lower your temp to "set" the pins but they should be raised again after a few days to high 70's or low 80's. Ameteurs, you do NOT need to do this, temperature changes are simply for fully orchestrated flushes and your mushroom patch will do just fine without such adjustments.

you will still get growth at 80 percent but you might get split or cracked caps - remember that there is a microclimate close to your casing that will keep the pins moist.

Click to expand...

Single FC. 2 clear shoebox size bins. Unknown species (oops, didn't label the bins when laying out). Sourced from 4 different possibilities.

Assume ALL are warm weather easy pinners that don't need wood.

Humidity varies between 95% and 100% (visible mist, hanging out, not condensing, even temp inside and out). That 80% was just for 1 night. My HEPA fan pushes outside air over the trays for 60 seconds every 10 minutes or so. I've got the lights at 6500 degree 30 watt CFLs on a 12/12 schedule, with occasional later on when I'm looking at them.

I can cool or heat the general environment very quickly to pretty much any extreme (40ish at night to 80+ anytime if wanted).

These are NOT cased. I do not care how many I get. Really, keep reading.

So the bin on the right is side and bottom pinning like crazy, with a few pins on top. This is expected and ok, I like it. The goal is simply to get a handful of clone material for the 50 jars in the ozone closet to feed the 10 prepped tubs on the wall.

The one on the left is dotted with primordia, and has been for about a week. It probably wants a bit of cold for the night. The one on the right would probably be a bit annoyed with cold for a night. Or not? You tell me (if you can).

Also, LOVE/HATE the All American Sterilizer. On a weighted PC, I set it on medium, the weight dances, I walk away for 2 hours. Since this thing is sealed, I heat until 15PSI, I attempt to set it, it ALWAYS creeps, I have to adjust, every 10 minutes. Or it either looses a shitload of pressure or hits the top, triggers the overload, and exhausts, dumping pressure too quick and drying the corn.

Grr

canndo said:

I believe it will germinate even in a near vaccum. Initialy your myclium needs very little oxygen and produced copious amounts of co2 (a bag is enough to change the ppm in a 10 x 10 room).

Then, Well, I don't know test. I have been working on mycelium storage for 20 years and have yet to find a satisfactory method.

Click to expand...

Well, it's been a couple of weeks. Out of 10 vacuum jars, 1 was knocked up with a GLC from a glow in the dark mushroom grain jar. The others were done with a karo MS LC, all dung loving warm easy pinners. Many more non-vacuum were done successfully from the same sources from the same needle on the same noc pass.

The ONLY vacuum one that grew was the cold/wood loving glow in the dark. Something tells me that bastard will grow on the outside of the space station. It is from myc stored in a needle in the garage that had NO protection for over a year. -20 to 110F.

None of the others grew at all, at least visibly. I will introduce air in a month or so and see if they start then.

Also, I left a regular jar with a GE filter in the ozone shock area the whole time. I did not shake it (forgot about it). It grew well. About a 1/3 colonized, strongly from the top. I have no worries about letting my jars incubate in a high ozone area to keep down the outside jar contaminants. On the other hand, it might eat the RTV. I'll pay attention.

maybe you need a new pressure regulator weight. i know i do.

V My bad. you don't i was just high being a douche

Skuxx said:

maybe you need a new pressure regulator weight. i know i do.

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Why? (10 chars)