I've got a corner area totally blocked out with hanging plastic staple gunned to the floor and gloves stuck through. Table and shelves on the other side, gets sealed with a zipper when loaded.
The "outside" area of at (where I'm standing) has plastic floor to ceiling, with a wraparound on the corner for me to step in.
Assume 100 SQ feet of floor, with 1/2 the norm air space due to sloped attic ceiling.
I do a decent clean prep. Oust and 10% bleach spray walls and air, and then I fry it with ozone.
The internal area (2x4) get a 360 mg/hour generator. That's the "clean area".
The external area (3x6, 3 x
gets a 200 mg/hour generator.
According to what's I've read (please correct me), these are "shock" amounts and should kill pretty much everything in the area in an hour or so. Do I need longer?
At that point I'll be as clean as possible for my PC unload, and unload it hot into the inner chamber. I could do a bunch of unloads over several days.
My next step is GLC procedure. Clean/introduce the source jar, sucking in sterile water, shooting it into source jar, shaking it up, sucking it out, inoculating a bunch of jars. Can this be done in the ozone rich environment, or do I have to turn it off for a while?
The target jars might have been sitting in the ozone rich area for a couple of days before use. Would it affect the top layer of air? Fry up the popcorn? They have synthetic disks glued to a dime size hole.
After the transfer, can I leave the jars in the ozone rich space? Should I turn it down but still leave them in? How does the ozone affect the myc as compared to everything else? I'm thinking I'll do both in and out of the area with the same GLC to see if there is a difference.
What about laying out substrate? Can I do it in this environment, or would that be bad? Can I leave the tubs in during grow/consolidate, or bad? What about fruiting? I know it is not sterile at that point, but would the lower germ load be helpful or harmful? Will the ozone permeate and kill everything, or will it sear the outer level and leave the inside alone?
The "outside" area of at (where I'm standing) has plastic floor to ceiling, with a wraparound on the corner for me to step in.
Assume 100 SQ feet of floor, with 1/2 the norm air space due to sloped attic ceiling.
I do a decent clean prep. Oust and 10% bleach spray walls and air, and then I fry it with ozone.
The internal area (2x4) get a 360 mg/hour generator. That's the "clean area".
The external area (3x6, 3 x
gets a 200 mg/hour generator.According to what's I've read (please correct me), these are "shock" amounts and should kill pretty much everything in the area in an hour or so. Do I need longer?
At that point I'll be as clean as possible for my PC unload, and unload it hot into the inner chamber. I could do a bunch of unloads over several days.
My next step is GLC procedure. Clean/introduce the source jar, sucking in sterile water, shooting it into source jar, shaking it up, sucking it out, inoculating a bunch of jars. Can this be done in the ozone rich environment, or do I have to turn it off for a while?
The target jars might have been sitting in the ozone rich area for a couple of days before use. Would it affect the top layer of air? Fry up the popcorn? They have synthetic disks glued to a dime size hole.
After the transfer, can I leave the jars in the ozone rich space? Should I turn it down but still leave them in? How does the ozone affect the myc as compared to everything else? I'm thinking I'll do both in and out of the area with the same GLC to see if there is a difference.
What about laying out substrate? Can I do it in this environment, or would that be bad? Can I leave the tubs in during grow/consolidate, or bad? What about fruiting? I know it is not sterile at that point, but would the lower germ load be helpful or harmful? Will the ozone permeate and kill everything, or will it sear the outer level and leave the inside alone?
