Uncle Ben's bags

Interesting, that mushrooms slowly degrade due to senescence, unlike plant clones. You can keep doing grain to grain transfers, which is way better than inoculating with spores, but eventually you'll need to culture new spores it sounds like... In other words, you could turn one bag of UB into as many as you want, without injecting each bag each time.

I might just do it that way, because doing 40 inoculations just sounds bad, even though i'm willing to gamble on it.. I'll be using laminar flow with hepa filter, and that would actually be easier to drop pieces of rice into the corner of each bag, then it would be to flame sterilize 2 syringes 20 times each.

Drop That Sound said:

Interesting, that mushrooms slowly degrade due to senescence, unlike plant clones. You can keep doing grain to grain transfers, which is way better than inoculating with spores, but eventually you'll need to culture new spores it sounds like... In other words, you could turn one bag of UB into as many as you want, without injecting each bag each time.

I might just do it that way, because doing 40 inoculations just sounds bad, even though i'm willing to gamble on it.. I'll be using laminar flow with hepa filter, and that would actually be easier to drop pieces of rice into the corner of each bag, then it would be to flame sterilize 2 syringes 20 times each.

Click to expand...

I only use a spore syringe once, when i'm after a new "strains" genetics . I go to agar to test for contam and once all is well with it i'll mix agar to popcorn. once I have 5th or 6th gen i'll take a print or swab and from there i never need another syringe of that strain again. I advocate g2g for simplicity and keeping it sterile af once you know it's not contammed

if you have a hood then fo sho use it, i used a nice home made still air box and 99% of the time never had any contam.

^ Sounds like the way to go!

My plan is to hit 2 agar dishes for each different syringe I got, (maybe even a slant, for long term storage in fridge) and then just squirt the rest into UB bags. That, and I heard you can squirt your MSS on some on foil, l and let it dry back out like a print again, for long term. It would probably dry pretty quick under a flow hood.

I know it would be better to do the MSS to agar first, or even test on a few bags, but i'm getting ancy and want to get my first grow going asap, so I'll I'll do both. I'm sure I'll get something good, and atleast one of the syringes I got must be a good culture.

Get this.. apparently you can take your almost (or even completely empty) spore syringe after you use it up, and poke it into a capri sun drink pouch, and suck up the juice. In a few weeks, if all goes well, you'll have a supercharged LC syringe of whatever spores you used.

You can inject directly into the capri sun pouches too.

While I have never used this method nor intend to..
.having been in the endeavor for some time I believe this may well be the easiest and cheapest method ever conceived.

Someone please post their experiences.

Drop That Sound said:

^ Sounds like the way to go!

My plan is to hit 2 agar dishes for each different syringe I got, (maybe even a slant, for long term storage in fridge) and then just squirt the rest into UB bags. That, and I heard you can squirt your MSS on some on foil, l and let it dry back out like a print again, for long term. It would probably dry pretty quick under a flow hood.

I know it would be better to do the MSS to agar first, or even test on a few bags, but i'm getting ancy and want to get my first grow going asap, so I'll I'll do both. I'm sure I'll get something good, and atleast one of the syringes I got must be a good culture.

Click to expand...

Wait... we can presume if one is working with agar then they needent bother with isolation. Just innoculate the bag with spores directly from the syringe, grow some fruit, select ones preference and take a tissue sample for the plate.


What could possibly be more simple and direct? Forget sectors, dish transfers, and repitition.

Of you actually want to go nuts, take a sample of different fruit from different times and compare those.

Converting tissue to an injectable product might be more involved but....not much really. Direct from tissue to liquid culture isn't all that tough and going lc to uncle Ben seems right straight forward.

My only real concern is the amount of plastic waste being created, which might not even be as bad as any other way though, not sure on that.. I don't think any of us could really produce anything as efficiently as a microwaveable rice pouch factory like they have tbh.

You still have to buy bulk rice/grains in a plastic bag anyway, if you decide to spend hours PC/broke boi'ing your own jars or bags. Dry rice doesn't look much cheaper than convenient ready to go bags either..

I can't help but wanting to go bigger right away, but it's just too many bags of rice to deal with. That's my other gripe.

Family size brown rice bags would be cool, and would technically cut down 50% of the work, but no brand has been making them yet. Not local to me anyway.. I'll pick up a large size UB white jasmine, and see what's up. I heard rice is slower, and it might take longer, meaning more chance of problems. So maybe the small 250 gram 90 sec bags are actually ideal?

I'm gonna attempt my first g2g with UB tek. Use 1 or 2 bags as the masters, and drop pieces into enough bags with corner cut GE holes to get the 16 full qts of spawn i'm after.

Gonna do it all in a clear trash bag with positive flow 99.99 hepa filtered air, lol. Talk about easy and cheap..