Ayuhuasca Purple, 260w HLG "Diablo", Sub Irrigated Probiotic Organics

BluntMoniker

Well-Known Member
The Basics
Breeder/Strain - Barney's Farm Ayuhuasca Purple (Fem)
Tent - 2x3x6
Light - HLG's 260w QB648 (2x diablo boards on a single heatsink) running at about 80% power @ 18in above canopy - First grow under the new light, switched over from a 250w HPS
Ventilation - 4in 180cfm inline fan/filter combo + 2x fans in tent to move air around

Soil Build
Base: 1 part Miracle Grow Performance Organic, 1 part Mushroom Compost, 2 parts Sunshine Mix Advance #4 (total of roughly 30gal base mixed)
Inputs: 2 cups Bio-Live, 1gal Fresh EWC, 1 cup Dolomite Lime, and a fat handful (roughly 1/2 cup) each of langbenite, seabird guano,blood meal, and azomite.
Building my own soil was a first as well. Based it loosely on several other people's recipes, but kept it fairly simple. Will likely switch from bio-live to BAS's craft blend once the bio-live is gone as it has a greater variety of inputs, but so far the bio-live has worked well.

Grow Style
Probiotic Organics in a Sub Irrigated 18gal tote. For more info on the grow style and how it works, please visit Green's Probiotic Method. I also revegged(monstercropped) the entire plant at 3weeks of flower. In total she was vegged for 4 weeks, put into flower for 3 weeks, put back into veg for 3 weeks, and is currently (as of 9/17) at exactly 5 weeks of flower.



(Week 1 veg) Here is the first picture I can find from this grow. Its a 1 week before and after, from the day I transplanted from my 1/2gal seedling pot into the 18gal SIP (sub irrigated pot). As you can see, growth was phenomenal:
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The white powder was DE (Diatomaceous Earth) as a preventative against soil mites. There were no mites (or so I thought) when I planted her in the SIP, but within days I saw THOUSANDS as well as spring tails jumping about all over. I simply sprinkled DE inside the SIP then kept it closed, and sprinkled DE on top of the SIP and the plant itself. Within a week or 2, all signs of bug activity had subsided. From what I was able to see with close ups, the mites were hypoasis miles mites, so I got lucky there, and while Im not 100% sure if they are still crawling around in my soil or not, they've neither moved UP my plant nor caused any negative issues throughout the grow, and having not seen them since I don't worry about it much.

(Week 3 veg) Here is 2 weeks later (week 3 of veg in the SIP). At this point, I had removed lower growth, she had been topped once, and I had started to use LST to spread her growth out:


Here is a pic of my average day/night temps/rH. Of course this is greatly effected by outdoor temps/rH, but i generally hover within these limits:
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(Week 1 Flower) This picture is 2 weeks after the last plant pic. At this point, shes at week 1 of her first round of flowering (pre reveg), and again growth is both extremely healthy and explosive. You can see its a before/after of having defoliated and further LSTed the plant to increase light coverage and use of my space:


(Week 1.5 flower) Here is one from several days later, after having put her under a homemade scrog net:


(Week 3 flower) This picture is roughly 2 weeks after the last (3 weeks of flower), and is the day I started my reveg. Knowing what I know now, I would have reverted back to veg a week sooner, because unbeknownst to me, the buds that you see formed here would continue to grow and swell as they normally would, which I feel would have been better avoided, or at least not allowed to progress as far as they did:


(Week 1 reveg) And now, at this point I've topdressed her once or twice with EWC, bloodmeal, and langbenite depending at the stage of growth. I didnt mark down the exact days I topdressed, and didnt want to guess so Im just lumping all that info here for anyone who cares to know. In this next picture, shes at 1 week of reveg. As I said previously, notice how buds continue to grow as you'd expect, regardless of the light cycle change having happened over a week ago:


(Week 2 reveg) Here is 1 week later, having been at 2 week back under veg lighting, and buds STILL continue to swell. BUT (and its hard to see it in this pic) new growth is JUST starting to form at the top of some buds. You can also see what I thought at the time, was odd leaf growths pushing out of the buds, which looked unlike sugar leaves to me.. again forming near the top of the buds:


(Week 2.5 reveg) Here is a closeup from several days later, with clear evidence that vegetative growth has begun to take over. Notice the almost foxtail-like growth of the bud itself with a single new growth tip pushing straight out of the top. Ive also included a few close ups of the new growth shoots growing out of individual calyxes. New growth is heavily dictated by bud orientation/light penetration, likely due to auxin movements:






(Week 3 reveg) Here are some pictures from the day I switched back to flower after reveg. Again, notice how large the original buds got (they never stopped growing), and how individual calyxes are growing off of their own stem and spreading, instead of what you'd normally see, which is calyxes compacting together as they swell. You can also see some purpling which is pretty sexy:



(Week 1 Final Flower) Here is 1 week after having switched from reveg to the "final flower" phase. All "original" buds were trained to stay below the net, while all new growth has been allowed to grow above the net to be sure it received ample light. You'll notice some yellow/brown leaves near the bottom, this was from letting the rez run dry accidentally... she went from needing 2gal of water a week, to 5gal a week seemingly over night!:



(Week 3 Final Flower) Here you can see buds forming nicely on the new growth, SCROG net almost completely full, and some purpling setting in on the new growth. You can see my WW clone in the corner peaking out. Thats just a semi-ignored plant in a 3gal container that I threw into the flower tent full time around this point. I dont plan on doing anything fancy with it, its just going to provide an extra 2-3oz in between the Ayuhuasca Purple, and my next SIP grow (likely be DoSiDo):


(Week 5 Final Flower) And here we are, up to date with where the grow is currently at. Again, ignore yellowing, because I let the rez run dry again >_>... Instead admire the beauty of the purples shes producing! The original buds are SUPER resinous, and the new growth is starting to swell up. Shes very fragrant and has a "purple" smell (for what thats worth) mixed with pine sol or some other piney/chemically smell. Overall Im very pleased with the strain so far, in terms of vitality, growth patterns, moderate stretch, tight nodal spacing, and hardiness:




Im going to try and update weekly from here on out (likely on Thursdays) until I harvest. Harvest date is estimated ROUGHLY as Oct 15th (9 weeks of flower) assuming everything goes according to plan. If you guys have any questions, love, or shit talking you wanna throw my way, feel free and ill get back to you when I can!
 

BluntMoniker

Well-Known Member
Maybe I missed it but, why did you decide to reveg? But, looks good man
It was something I had read in one of @Richard Drysift 's threads, and decided I wanted to try it out. If I can get him in here, he can explain a bit better than I can as far as the process and how it works.

I did it a bit differently than he does, as he uses gradual light changes where I did a straight 12/12 to 20/4 and then back. Essentially though, when I originally read about it, I took it as "this will increase chunkiness of your buds", which is why I did it.

With that said, my best ever yield out of my 2x3 was with 4x 5gal buckets under a 250w HPS (8oz~, roughly 2oz per plant).. so yield is going to tell the tale of how worthwhile revegging was. It added a solid 6 weeks to my overall grow time, so if it doesn't produce at least a good 10-12oz I likely will only try it once more...

This is also the first grow under the new light, and using the probiotic method, so any/all of that is going to play a factor, which is why I'd be willing to give it a second go around, depending.
 

Richard Drysift

Well-Known Member
Increasing the chunky-ness of the buds is a good way to explain it actually. Light manipulation or monster cropping are more popular terms but essentially increasing bud mass is the goal. Most growers switch from a veg phase light cycle to a flowering light cycle to trigger buds to form but the sun does not do it like this in nature. The suns light cycle changes gradually over the spring & summer then into fall. You usually don’t see stretched out lanky ass plants outdoors; they are typically chunky with fat colas unless deprived of sunlight. Fat colas are the result of a gradual switch into flowering.
I’ve been experimenting with light manipulation to increase yields in my perpetual grow. I have found if you just do a standard monster crop...i.e. flipping from 18/6 to 12/12 for a couple weeks and then reveg back to 18/6 for a couple more weeks... bud mass is increased. However if you go even further and begin dropping the hours of daylight by let’s say an hour or two per week the increase of bud mass is even more defined. When they get to about 14/10 most strains will throw pistils. You can get the plants to a place where they are vegging and blooming at the same time. This will make some chunky ass bud. Only trouble is with growing big ass bud you can end up with other problems like mold but if you have adequate lighting, extra space and time the yields are worth the effort.
 

SpawnOfVader

Well-Known Member
Increasing the chunky-ness of the buds is a good way to explain it actually. Light manipulation or monster cropping are more popular terms but essentially increasing bud mass is the goal. Most growers switch from a veg phase light cycle to a flowering light cycle to trigger buds to form but the sun does not do it like this in nature. The suns light cycle changes gradually over the spring & summer then into fall. You usually don’t see stretched out lanky ass plants outdoors; they are typically chunky with fat colas unless deprived of sunlight. Fat colas are the result of a gradual switch into flowering.
I’ve been experimenting with light manipulation to increase yields in my perpetual grow. I have found if you just do a standard monster crop...i.e. flipping from 18/6 to 12/12 for a couple weeks and then reveg back to 18/6 for a couple more weeks... bud mass is increased. However if you go even further and begin dropping the hours of daylight by let’s say an hour or two per week the increase of bud mass is even more defined. When they get to about 14/10 most strains will throw pistils. You can get the plants to a place where they are vegging and blooming at the same time. This will make some chunky ass bud. Only trouble is with growing big ass bud you can end up with other problems like mold but if you have adequate lighting, extra space and time the yields are worth the effort.
EDIT: should double read large text blocks when smoking- disregard

Do you have any side by side studies on doing this vs not? (Not doubting, just curious about what the metrics are on the difference)
 

SpawnOfVader

Well-Known Member
EDIT: should double read large text blocks when smoking- disregard

Do you have any side by side studies on doing this vs not? (Not doubting, just curious about what the metrics are on the difference)
The gradual change totally makes sense- if I had two full tents going right now I'd test just altering the schedule an hour at a time over a period vs not with some clones.
 

Richard Drysift

Well-Known Member
EDIT: should double read large text blocks when smoking- disregard

Do you have any side by side studies on doing this vs not? (Not doubting, just curious about what the metrics are on the difference)
I should add that all this is not my idea; It was penned by the Rev in a back issue of skunk magazine. I don’t do side by side for studies; just a home hobby grower here trying to get the most out of my investment. If you go to the end of my thread here you can see the results for better or worse...


The reason I started doing this besides the obvious increase of yield was because I had plants in veg awaiting available space in my dedicated bloom room. Was thinking it would slow them down while increasing the amount of nodal areas where buds grow. Ended up with these nice big corn cob shaped buds. So I told everyone that would listen to go try it..
 

SpawnOfVader

Well-Known Member
I should add that all this is not my idea; It was penned by the Rev in a back issue of skunk magazine. I don’t do side by side for studies; just a home hobby grower here trying to get the most out of my investment. If you go to the end of my thread here you can see the results for better or worse...


The reason I started doing this besides the obvious increase of yield was because I had plants in veg awaiting available space in my dedicated bloom room. Was thinking it would slow them down while increasing the amount of nodal areas where buds grow. Ended up with these nice big corn cob shaped buds. So I told everyone that would listen to go try it..
That's a situation I could definitely see myself running into. If that happens I'll definitely give this a shot. Thanks for sharing the link for the article, I'll read through it!
 

BluntMoniker

Well-Known Member
That's a situation I could definitely see myself running into. If that happens I'll definitely give this a shot. Thanks for sharing the link for the article, I'll read through it!
Yea, and my early observations are increased node count, increased nodal stacking, and then the swell on the buds being increased as well. Thats all just observation without exact numbers backing it, but so far the reveg seems to have not only increased node size/count but created a hardier plant in terms of root stock/branch strength.

But again, the numbers will tell the tale lol
 

SpawnOfVader

Well-Known Member
Yea, and my early observations are increased node count, increased nodal stacking, and then the swell on the buds being increased as well. Thats all just observation without exact numbers backing it, but so far the reveg seems to have not only increased node size/count but created a hardier plant in terms of root stock/branch strength.

But again, the numbers will tell the tale lol
My first project once I get my commercial op up and going is to have two small identical spaces so we can finally settle some of these disputes with empirical evidence.
 

BluntMoniker

Well-Known Member
My first project once I get my commercial op up and going is to have two small identical spaces so we can finally settle some of these disputes with empirical evidence.
That would be awesome. Really, you should do 3 clones... 1 with no reveg (just extend the original veg time), one with a reveg that uses gradual light changes like Dick Drysift does, and 1 with a reveg more like mine that goes straight to 12/12 and back again.

Then we can really see not only is revegging worth it, but then which form of revegging produces what, in terms of quantity, quality, shape/structure, etc
 

BluntMoniker

Well-Known Member
(Week 6 Final Flower) Not much has gone on this week. I did some minor rearranging of the tops to better expose bud sites lower down, but otherwise were just cruising along to the end.

Here are a couple update pics, one is lights off w/ flash, the other is lights on:




You can see my White Widow in her 3 gal pot in the corner. Shes barely visible, but a good color reference to compare against the extreme purple/pink tones of the Ayuhuasca Purple.
 

BluntMoniker

Well-Known Member
(Week 7 Final Flower) Got a few update pics as we move into the final week or 2 of flower. I plan on pulling her this time next week, but may go an additional week depending on how much smoke I have left from my last round bongsmilie.

Shes noticeably swelling and adding heavy frost, buds themselves are turning purple (not just the leaves anymore), trichs appear to be mostly milky w/ a few ambers here or there and a few clears left here and there. Water intake has slowed way down as well. Shes gone from needing roughly 3 gallons per week in the rez, to barely sucking up 1-1.5. She is definitely nearing her end time, and I couldn't be more excited, as she looks and smells amazing. Fairly happy so far with not only this strain (and its tolerance to my fuck ups lol), and @Greenthumbs256 Probiotic method.

I feel next time, Id skip the re-veg, and try to stick more closely to Dick Drysift's method of reveg if I were to consider it again. The way I did it, the lower bud growth, which looks like it will be resinous as hell, is going to end up airy once dried simply because of the funky growth patterns causing calyxes to push outward before swelling, where normally calyxes stay compact on the flower during the swell. Either way, From the look of it Im in for a good harvest weight, and a killer product so im happy!


Lights out, flash on shot of one of the colas, just for frost reference.


Overall shot of the plant. Looking at the pattern of purpling, it appears that the light intensity played some factor in what turned purple and what didnt. Colas and leaves above the net took on a HEAVY purpling effect, while buds/growth below the net do have some purpling there is also still some green buds/leaves. I've also noticed yellows/pinks which I'm attributing to under nute-ing when I made my soil mix, but luckily enough Im close enough to the end that it shouldn't matter TOO much.


Just a few more pics. 1 is a picture of "old growth" buds, distringuished by the bulbous calyxes spread out across the branches, the other is "new growth" showing several colas that grew out of old growth calyxes during the reveg process. Again, the odd growth patterns, while fun to watch and play with, are not something Im interested in for future grows unless I were to tweak my methodology


 

BluntMoniker

Well-Known Member
I'm finding that some of the old growth buds that are lower down on the plant, are having the outer leaves of the calyxs brown and die off, similar to what im seeing in regular leaves.

Removed 2 buds just to inspect them further... the stems themselves are green, and the interior of the buds are green, no sign of it being rot,

Its almost as if the plant is treating the old growth buds as if they are leaves. Or at least, the old growth buds that receive the least amount of light are. Old growth buds that receive direct light up near the top of the canopy are dense and extremely resinous, while these lower areas are more airy/wispy.

Just another interesting observation made in regards to the reveg ive done this round
 

BluntMoniker

Well-Known Member
(Week 8 Final Flower) Just some update pics. I posted yesterday about outer calyx leaves dying off, so I took some pictures to show what Im talking about and give a visual. Also took some Trichome shots.. Id say its ROUGHLY 20% clear, 75% milky, 5% amber as of right now, but here's some of the better pics I was able to get

Normal calyxs (purple/green)


Browning calyx


Trich Shots


 

BluntMoniker

Well-Known Member
Oh shit, I completely forgot to finish this one out.

I'll search around later and see if I have post harvest shots laying around, otherwise you guys (all 2 of the people who actually scrolled this far :p) will have to be happy with just the final stats:

Harvest Date: 10/13 (Day 62)
Final Weight: 204.8 (7.314oz)
111.1g (3.96oz) Jar quality nug
93.7g (3.35oz) larf
Smoke Report: The high itself was a good mix of head/body high. Has a very piney taste up front, with notes of chemical and fruit. Overall a pleasant all around smoke for morning, noon, or night.


I dont think doing a reveg in that form will be something I do again. I may try something closer to Dick Drysifts method of slowly changing light cycle back and fourth one day, but with the way this run turned out, it wont be soon.

The buds that formed on main stems did not grow like any buds you've seen before. Imagine taking corn kernels off the cob by hand, and this is the closest thing I can use to describe the process of removing buds that grow on the main stems, or had main stems growing out of them.

The odd growth accounted for the reason almost half of my yield was "larf". I ended up using 85g+- for BHO, and ended up with 15.1g (17.5%+-) yield, which after winterizing and decarbing Ill use to make myself some vape carts. I've made several so far using Wax Liquidizer, and they came out great (would recommend the Liquidizer product).

Otherwise, the Ayuhuasca Purple was a fun and fairly easy grow.
 
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